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Western blot - JMY antibody (ab69945)
(enlarge)
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Product Name
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JMY antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JMY
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Immunogen
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Synthetic peptide derived from an internal sequence of human JMY.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab69945 detects endogenous levels of total JMY protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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COLO cell extracts.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
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Relevance
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JMY, junction mediating and regulatory protein, is a cofactor that increases p53/TP53 response via its interaction with p300/EP300. It also increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab69945 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JMY antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JMY antibody images, references, products related to ab69945 and other tools.
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JMY antibody images:
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 Western blot - JMY antibody (ab69945)
All lanes : JMY antibody (ab69945) at 1/500 dilution
Lane 1 : COLO cell exctract
Lane 2 : COLO cell exctract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 112 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to JMY
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JMY antibody - more information
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Customer reviews (feedback) regarding JMY antibody |
Customer FAQs regarding JMY antibody |
Protocols for JMY antibody |
Price and availability of products related to JMY antibody |
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JMY antibody - related products:
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JMY antibody - other tools:
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Contact Abcam with a Technical Enquiry about JMY antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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