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Product name
Anti-JNK1+JNK2 (phospho T183 + Y185) antibody
See all JNK1+JNK2 products (6) ...
Description
Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185)
Specificity
Phosphorylation site-specific antibody selective for the dually phosphorylated form of the c-Jun N-terminal Kinase (JNK)/Stress-Activated Protein Kinase (SAPK) enzymes containing a phosphate on threonine 183 and tyrosine 185 (human JNK 1 + 2). The antibody has been shown to recognize the endogenous, active forms of JNK 1 + 2 in a variety of cell types following treatment by a broad range of extracellular stimuli [e.g. including 293 cells (human embryonic kidney; +/- ultraviolet light) and PC12 cells (rat pheochromocytoma; +/- sorbital)].
Tested applications
ICC/IF, IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
a wide range of other species
Immunogen
Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence. This region is conserved among many species including human, rat, mouse, chick, nematode (Caenorhabditis elegans), and fly (Drosophila melanogaster).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK enzymes. The final product is generated by affinity chromatography using a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185, within the activation loop. Note: It is the dually phosphorylated form of these enzymes that has full enzymatic activity.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/1 - 1/100. 1/100.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 49, 55 kDa.
Target
Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Target information above from: UniProt accessionP45983
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c jun N terminal kinase 2 antibody
- cJun N terminal kinase 1 antibody
- c Jun kinase 2 antibody
see all
Database links
- Entrez Gene: 5601 Human
- Entrez Gene: 5599 Human
- Entrez Gene: 26420 Mouse
- Entrez Gene: 26419 Mouse
- Omim: 602896 Human
- Omim: 601158 Human
- SwissProt: P45984 Human
- SwissProt: P45983 Human
see all
Anti-JNK1+JNK2 (phospho T183 + Y185) antibody images:
Western blot - JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821)
Predicted band size : 49, 55 kDa
To demonstrate the phosphorylation of JNK 1 & 2 in a cell based assay, 293 cells were treated with ultraviolet irradiation (UV). Proteins from cell extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were incubated with either 1 µ g/mL ab4821 or 1 µg/mL anti-JNK1 pan. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method.
- JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821)
Figure shows immunostaining of fibroblast podosomes using ab4821. Green = dual phospho JNK, Red = mitochondria, Blue = nucleus.
Immunocytochemistry/ Immunofluorescence - JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821)
ab4821 staining JNK1+JNK2 (phospho T183 + Y185) in human foreskin fibroblasts by ICC/IF. The cells were fixed in cytoskeletal fixative, permeabilized in 0.5% Triton X-100 and blocked in 2% dillution buffer (2%BSA + 0.1% Triton X-100) for 1 hour at 25°C. The primary antibody was diluted, 1/100 and incubated with sample for 12 hours. An Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG, diluted 1/250 was used as secondary.
This image is courtesy of an Abreview submitted by Mr George Chennell
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821)
ab4821 staining JNK1+JNK2 (phospho T183 + Y185) in E12.5 mouse heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent cold 1% paraformaldehyde fixation before heat mediated antigen retrieval with Tris-EDTA pH9 and then blocking with 2.25% horse serum was performed for 20 minutes at RT. The primary antibody was diluted 1/400 and incubated with sample for 12 hours at 4°C. A Biotin conjugated horse polyclonal to rabbit IgG was used at dilution at 1/133 as secondary antibody.
This image is a courtesy of Michelle Combs
References for Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab4821)
This product has been referenced in:
- Combs MD & Yutzey KE VEGF and RANKL regulation of NFATc1 in heart valve development. Circ Res 105:565-74 (2009).Read more (PubMed: 19661463) »
- Viejo-Borbolla A et al. Enhancement of chemokine function as an immunomodulatory strategy employed by human herpesviruses. PLoS Pathog 8:e1002497 (2012). WB; Human.Read more (PubMed: 22319442) »
See all 6 publications for this product
Publishing research using ab4821? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"