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Product name
Anti-JNK1 antibody
See all JNK1 products (17) ...
Description
Rabbit polyclonal to JNK1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human).
Positive control
HEK293 +/- UV irradiation and PC12 cells +/- sorbitol treatment.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, 1mg/ml BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by epitope-specific affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
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Western blot - JNK1 antibody (ab10664)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab10664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 46 kDa.
Target
Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Target information above from: UniProt accessionP45983
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI849689 antibody
- c Jun N terminal kinase 1 antibody
- C-JUN kinase 1 antibody
see all
Database links
- Entrez Gene: 5599 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 116554 Rat
- Omim: 601158 Human
- SwissProt: P45983 Human
- SwissProt: Q91Y86 Mouse
- SwissProt: P49185 Rat
- Unigene: 138211 Human
see all
Anti-JNK1 antibody images:
Western blot - JNK1 antibody (ab10664)
Predicted band size : 46 kDa
Extracts prepared from HEK 293 cells not treated (-) or treated (+) with 90mJoules UV irradiation for 30 seconds were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were incubated with either 0.50 µg/mL JNK1&2 [pTpY183/185] antibody (A) or 1.0 µg/mL ab10664 (B). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected by chemiluminescence. The data show induction of activation after UV treatment, and the total level of JNK1 present.
Protocols
References for Anti-JNK1 antibody (ab10664)
This product has been referenced in:
- Spurlock CF et al. Increased sensitivity to apoptosis induced by methotrexate is mediated by jun N-terminal kinase. Arthritis Rheum : (2011).Read more (PubMed: 21618198) »
- Cook PJ et al. Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Nature 458:591-6 (2009).Read more (PubMed: 19234442) »
See all 3 publications for this product
Publishing research using ab10664? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"