Anti-Jagged1 antibody [EPR4290] (ab109536)

Overview

• Product nameAnti-Jagged1 antibody [EPR4290]See all Jagged1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4290] to Jagged1
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues near the C-terminus of Human Jagged1.

• Positive controlHepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at -20ºC.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4290
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Notch
  • Surface Molecules

  • Stem Cells
  • Hematopoietic Progenitors
  • Surface Molecules

  • Cardiovascular
  • Angiogenesis
  • Inhibitors

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Neurology process
  • Notch Pathway

Applications

Target

• FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
• Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
• Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
  Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
• Sequence similaritiesContains 1 DSL domain.
  Contains 15 EGF-like domains.
• Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
• Cellular localizationMembrane.

Target information above from: UniProt accession P78504 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 182 Human
  • Entrez Gene: 16449 Mouse
  • Entrez Gene: 29146 Rat
  • Omim: 601920 Human
  • SwissProt: P78504 Human
  • SwissProt: Q9QXX0 Mouse
  • SwissProt: Q63722 Rat
  • Unigene: 224012 Human

  • Unigene: 22398 Mouse
  • Unigene: 88804 Rat

  see all

• Alternative names
  AGS antibodyAHD antibodyAWS antibody

  CD 339 antibodyCD339 antibodyCD339 antigen antibodyHeadturner antibodyhJ1 antibodyHtu antibodyJag 1 antibodyJag1 antibodyJAG1_HUMAN antibodyJagged 1 antibodyJagged1 (Alagille syndrome) antibodyJagged1 antibodyJAGL1 antibodyMGC104644 antibodyOTTHUMP00000030278 antibodyProtein jagged-1 antibodySer 1 antibodySer1 antibodySerrate 1 antibodySlalom antibody

  see all

Anti-Jagged1 antibody [EPR4290] images

• 

  Western blot - Jagged1 antibody [EPR4290] (ab109536)
  All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : NIH:OVCAR-3 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 134 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Jagged1 antibody [EPR4290] (ab109536)
  ab109536 at 1/250 dilution staining Jagged1 in paraffin-embedded bladder cancer tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Jagged1 antibody [EPR4290] (ab109536)
  ab109536 at 1/100 dilution staining Jagged1 in HeLa cells by Immunofluorescence.