Overview

  • Product nameAnti-Jagged1 antibody [EPR4290]See all Jagged1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4290] to Jagged1
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of Human Jagged1.

  • Positive control
    • HepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 134 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
    • Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
    • Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
    • Sequence similaritiesContains 1 DSL domain.
      Contains 15 EGF-like domains.
    • Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P78504 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AGS antibody
      • AHD antibody
      • AWS antibody
      • CD 339 antibody
      • CD339 antibody
      • CD339 antigen antibody
      • Headturner antibody
      • hJ1 antibody
      • Htu antibody
      • Jag 1 antibody
      • Jag1 antibody
      • JAG1_HUMAN antibody
      • Jagged 1 antibody
      • Jagged1 (Alagille syndrome) antibody
      • Jagged1 antibody
      • JAGL1 antibody
      • MGC104644 antibody
      • OTTHUMP00000030278 antibody
      • Protein jagged-1 antibody
      • Ser 1 antibody
      • Ser1 antibody
      • Serrate 1 antibody
      • Slalom antibody
      see all

    Anti-Jagged1 antibody [EPR4290] images

    • All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : NIH:OVCAR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 134 kDa
    • ab109536 at 1/250 dilution staining Jagged1 in paraffin-embedded bladder cancer tissue by Immunohistochemistry.
    • ab109536 at 1/100 dilution staining Jagged1 in HeLa cells by Immunofluorescence.

    References for Anti-Jagged1 antibody [EPR4290] (ab109536)

    This product has been referenced in:
    • Yang W  et al. Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature 499:491-5 (2013). Mouse . Read more (PubMed: 23863940) »
    • Yoshida R  et al. The pathological significance of Notch1 in oral squamous cell carcinoma. Lab Invest 93:1068-81 (2013). Human . Read more (PubMed: 23938602) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109536.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"