Anti-Jagged1 antibody [MM0424-4A24] (ab89663)

Overview

  • Product nameAnti-Jagged1 antibody [MM0424-4A24]See all Jagged1 primary antibodies ...
  • Description
    Mouse monoclonal [MM0424-4A24] to Jagged1
  • Tested applicationsWB, Flow Cyt more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length Human Jagged1 protein.

  • EpitopeThe epitope is extracellular.

Properties

Applications

Our Abpromise guarantee covers the use of ab89663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
Flow Cyt 1/50 - 1/200. Extracellular epitope -- no need to permeabilize.

Target

  • FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P78504 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

References for Anti-Jagged1 antibody [MM0424-4A24] (ab89663)

ab89663 has not yet been referenced specifically in any publications.

Product Wall

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. I appreciate your patience while I was contacting the testing lab regarding your inquiry.

The lab confirmed t...

Read More

Thank you for contacting Abcam regarding ab89663.


I am sorry that you have been experiencing difficulties with this antibody in FACS. The immunogen is extra cellular and it is not necessary to permeabilize cells for FC. Based on the pro...

Read More

Thank you for your enquiry. This antibody has been used for Flow cytometry in human endothelial cells with 2.5ug of antibody for 1x10(6) cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"