Overview

  • Product nameAnti-Jagged1 antibodySee all Jagged1 primary antibodies ...
  • Description
    Goat polyclonal to Jagged1
  • Tested applicationsELISA, WB, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant rat jagged-1 extracellular domain expressed in mouse NSO cells.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 146 kDa.
Neutralising Use a concentration of 2 - 10 µg/ml. For neutralization of receptor-ligand interaction, a working concentration of 2-10 ug/ml is recommended. The antibody will block 50% of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 ul of a 5 ug/ml solution coated in each well) in an ELISA assay.
Flow Cyt Use a concentration of 20 µg/ml.

Target

  • FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • HJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser-1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Serrate-1 antibody
    • Slalom antibody
    see all

References for Anti-Jagged1 antibody (ab10580)

This product has been referenced in:
  • Radke AL  et al. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood 113:3092-101 (2009). Electron Microscopy, Flow Cyt ; Human . Read more (PubMed: 19171875) »

See 1 Publication for this product

Product Wall

Thank you for your patience. Regarding ab10580 (Goat polyclonal to Jagged 1), this is a polyclonal antibody therefore it is likely that several isotypes are present. However, this product has not been assayed to determine what isotypes are present....

Read More

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse h...

Read More

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has ...

Read More

Thank you for your enquiry. To our knowledge this antibody has yet to be tested mouse samples for FACS. The only tested species are those listed on our datasheet. We do not routinely offer free or trial sized samples for testing purposes. Please take i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"