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Recombinant rat jagged-1 extracellular domain expressed in mouse NSO cells.
Our Abpromise guarantee covers the use of ab10580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 146 kDa.|
|Neutralising||Use a concentration of 2 - 10 µg/ml. For neutralization of receptor-ligand interaction, a working concentration of 2-10 ug/ml is recommended. The antibody will block 50% of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 ul of a 5 ug/ml solution coated in each well) in an ELISA assay.|
|Flow Cyt||Use a concentration of 20 µg/ml. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"