Anti-Jagged1 antibody (ab10580)
- Product nameAnti-Jagged1 antibodySee all Jagged1 primary antibodies ...
- DescriptionGoat polyclonal to Jagged1
- Tested applicationsELISA, WB, Neutralising, Flow Cyt more details
- Species reactivityReacts with: Rat, Human
Recombinant rat jagged-1 extracellular domain expressed in mouse NSO cells.
- General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThe antibody is purified using rat jagged 1 affinity chromatography.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab10580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||ELISA: Use a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well.|
|WB||WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 146 kDa.|
|Neutralising||Neut: Use a concentration of 2 - 10 µg/ml. For neutralization of receptor-ligand interaction, a working concentration of 2-10 ug/ml is recommended. The antibody will block 50% of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 ul of a 5 ug/ml solution coated in each well) in an ELISA assay.|
|Flow Cyt||Flow Cyt: Use a concentration of 20 µg/ml.|
- FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
- Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
- Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
- Sequence similaritiesContains 1 DSL domain.
Contains 15 EGF-like domains.
- Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
- Cellular localizationMembrane.
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References for Anti-Jagged1 antibody (ab10580)
This product has been referenced in:
- Radke AL et al. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood 113:3092-101 (2009). Electron Microscopy, Flow Cyt ; Human . Read more (PubMed: 19171875) »