Overview

  • Product nameAnti-Jagged1 antibody
    See all Jagged1 primary antibodies
  • Description
    Rabbit polyclonal to Jagged1
  • Tested applicationsIHC-FoFr, ICC/IF, IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1

Properties

Applications

Our Abpromise guarantee covers the use of ab7771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
IHC-P 1/100.
WB 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate and a 75 kDa band in human brain and kidney lysates.
IHC-Fr 1/100.

Target

  • FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • HJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser-1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Serrate-1 antibody
    • Slalom antibody
    see all

Anti-Jagged1 antibody images



  • Predicted band size : 134 kDa


    ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
    Exposure time: 1 minute. Expected molecular weight: 134 kDa

    Lanes 1 to 3 and 5: 20µg of cell lysate per lane
    Lane 4: 20 µl of 1x LDS loading buffer

    Lane 1: Human Brain tissue lysate
    Lane 2: Human Kidney tissue Lysate
    Lane 3: Human liver tissue lysate
    Lane 4: 1x LDS loading buffer
    Lane 5: Mouse liver lysate

  • Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).

  • Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin

  • Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin

References for Anti-Jagged1 antibody (ab7771)

This product has been referenced in:
  • Campese AF  et al. Mouse Sertoli cells sustain de novo generation of regulatory T cells by triggering the notch pathway through soluble JAGGED1. Biol Reprod 90:53 (2014). WB . Read more (PubMed: 24478388) »
  • Chao CH  et al. MicroRNA-205 signaling regulates mammary stem cell fate and tumorigenesis. J Clin Invest 124:3093-106 (2014). IHC . Read more (PubMed: 24911147) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Cell (Breast cancer cell line)
Specification Breast cancer cell line
Permeabilization Yes - 0.1% Triton-X in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 16 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Sample Cow Tissue sections (Liver)
Specification Liver
Permeabilization Yes - Triton X
Fixative Formaldehyde
Username

Dr. Daniel Murta

Verified customer

Submitted Jun 07 2013

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the Anti-Jagged1 antibody (ab7771) .

I would like to reassure you that this antibody is tested and covered by our 6 mon...

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I have heard back for the supplying lab.

The IHC data we have on the datasheet were contributed by their endusers. Unfortunately, they do not have a specific protocol available and all conditions must be user optimized.

I am sorry to ...

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Thank you for your reply and the image.

In the image I am not quite able to tell where the nuclei or potential plasma membranes are as there is no reference point. Does the image reflect just 1 cell or a line up of cells?
Would you have an...

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Thank you for contacting us.

I am checking with the lab regarding the protocol, but could you please send me in the meantime an image showing the staining you are obtaining?

I look forward to your reply and helping you further.
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking time tocontact us. I am sorry to hear that this antibody is not providing satisfactory results.

Are the samples you are staining the same as you have tried previously?

What dilutions of the antibody have you t...

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Thank you for your reply. For Jagged1, I would recommend ab7771, as it guaranteed to work in IHC-P on mouse tissue. As for the positive control, according to www.proteinatlas.org, Jagged1 is highly expressed in liver and pancreas, there is also stainin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Breast gland)
Specification Breast gland
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Rene Villadsen

Verified customer

Submitted Oct 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"