Overview

  • Product nameAnti-Jagged1 antibodySee all Jagged1 primary antibodies ...
  • Description
    Rabbit polyclonal to Jagged1
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human Jagged1.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MEL-1; HeLa; HEK293

Properties

Applications

Our Abpromise guarantee covers the use of ab85763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC/IF
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionLigand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
    • Tissue specificityWidely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
    • Involvement in diseaseDefects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
    • Sequence similaritiesContains 1 DSL domain.
      Contains 15 EGF-like domains.
    • Developmental stageExpressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P78504 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AGS antibody
      • AHD antibody
      • AWS antibody
      • CD 339 antibody
      • CD339 antibody
      • CD339 antigen antibody
      • Headturner antibody
      • HJ1 antibody
      • Htu antibody
      • Jag 1 antibody
      • Jag1 antibody
      • JAG1_HUMAN antibody
      • Jagged 1 antibody
      • Jagged1 (Alagille syndrome) antibody
      • Jagged1 antibody
      • JAGL1 antibody
      • MGC104644 antibody
      • OTTHUMP00000030278 antibody
      • Protein jagged-1 antibody
      • Ser 1 antibody
      • Ser-1 antibody
      • Ser1 antibody
      • Serrate 1 antibody
      • Serrate-1 antibody
      • Slalom antibody
      see all

    Anti-Jagged1 antibody images

    • All lanes : Anti-Jagged1 antibody (ab85763) at 1 µg/ml

      Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 134 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 105 kDa,40 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes
    • ICC/IF image of ab85763 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • ICC/IF image of ab85763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Jagged1 antibody (ab85763)

    This product has been referenced in:
    • Cuman C  et al. Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success. Hum Reprod 28:1161-71 (2013). Human . Read more (PubMed: 23477906) »

    See 1 Publication for this product

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