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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Jarid2 antibody
See all Jarid2 products (3) ...
Description
Rabbit polyclonal to Jarid2
Specificity
This antibody is specific for Jarid2
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
A synthetic peptide made to an N-terminal portion of the human Jarid2 protein, between residues 1-100
Positive control
E16 mouse brain lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Tris glycine, 150mM Sodium chloride
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Jarid2 antibody (ab48137)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
Target
Function
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
Tissue specificity
During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
Sequence similarities
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Domain
The ARID domain is required to target the PRC2 complex to its target genes.
The GSGFP motif is required for the interaction with SUZ12.
Cellular localization
Nucleus. Colocalizes with the PRC2 complex on chromatin.
Target information above from: UniProt accessionQ92833
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JARD2 antibody
- JARD2_HUMAN antibody
- JARID2 antibody
see all
Database links
- Entrez Gene: 3720 Human
- Entrez Gene: 16468 Mouse
- Omim: 601594 Human
- SwissProt: Q92833 Human
- SwissProt: Q62315 Mouse
- Unigene: 269059 Human
- Unigene: 25059 Mouse
Anti-Jarid2 antibody images:
Western blot - Jarid2 antibody (ab48137)
Anti-Jarid2 antibody (ab48137) at 2 µg/ml + E16 mouse brain lysate at 50 µg
Predicted band size : 139 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa,80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Jarid2 antibody (ab48137)
This product has been referenced in:
- Wu Het al. Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells. Nature : (2011).Read more (PubMed: 21451524) »
- Landeira Det al. Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol 12:618-24 (2010). WB; Mouse.Read more (PubMed: 20473294) »
See all 2 publications for this product
Publishing research using ab48137? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"