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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunB antibody
See all JunB products (9) ...
Description
Rabbit polyclonal to JunB
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Carp, Zebrafish
Immunogen
Synthetic peptide: TKMEQPFYHDDSYTA, corresponding to amino acids 3-17 of Human JunB.
TKMEQPFYHD DSYTA
Positive control
3T6 Swiss albino nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal
Constituents: PBS containing 0.2% Gelatin
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - JunB antibody (ab11981)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
Target
Relevance
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: P17275 Human
- SwissProt: P09450 Mouse
- SwissProt: P24898 Rat
- Unigene: 25292 Human
see all
Anti-JunB antibody images:
Western blot - JunB antibody (ab11981)
Predicted band size : 38 kDa
Detection of JunB by Western blot analysis. Antibody titration experiments using JunB rabbit polyclonal antibody in nuclear extracts derived from 3T6 Swiss Albino cells (lanes 1 to 3). The signal corresponding to nuclear JunB is eliminated when the immunizing peptide is added to the reaction mixture
References for Anti-JunB antibody (ab11981)
This product has been referenced in:
- Gutierrez GJet al. Interplay between Cdh1 and JNK activity during the cell cycle. Nat Cell Biol 12:686-95 (2010). WB; Human.Read more (PubMed: 20581839) »
See 1 publication for this product
Publishing research using ab11981? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"