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Western blot - JunB antibody (ab11981)
(enlarge)
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Product Name
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JunB antibody
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See all JunB antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JunB
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Immunogen
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Synthetic peptide: TKMEQPFYHDDSYTA, corresponding to amino acids 3-17 of Human JunB.
BLAST 'TKMEQPFYHDDSYTA' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
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Rat, Carp, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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3T6 Swiss albino nuclear extract.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge.
JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal
Constituents: PBS containing 0.2% Gelatin Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JunB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JunB antibody images, references, products related to ab11981 and other tools.
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JunB antibody images:
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 Western blot - JunB antibody (ab11981)
Predicted band size : 38 kDa
Detection of JunB by Western blot analysis. Antibody titration experiments using JunB rabbit polyclonal antibody in nuclear extracts derived from 3T6 Swiss Albino cells (lanes 1 to 3). The signal corresponding to nuclear JunB is eliminated when the immunizing peptide is added to the reaction mixture
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Search PubMed (MEDLINE) for references to JunB
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JunB antibody - more information
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Customer reviews (feedback) regarding JunB antibody |
Customer FAQs regarding JunB antibody |
Protocols for JunB antibody |
Price and availability of products related to JunB antibody |
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JunB antibody - related products:
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JunB antibody - other tools:
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Contact Abcam with a Technical Enquiry about JunB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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