Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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ab31421 has been referenced in 3 publications.
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ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : Un-treated.
Right image : Antibody pre-incubated with synthesized peptide.
All lanes : Anti-JunB antibody (ab31421) at 1/500 dilution
Lane 1 : Extracts of Hela cells.
Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.
Predicted band size : 35 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab31421 stained Hek293 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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