|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - JunB antibody (ab31421)
(enlarge)
|
|
Western blot - JunB antibody (ab31421)
(enlarge)
|
|
|
|
|
Product Name
|
JunB antibody
|
| |
See all JunB antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to JunB
|
|
Immunogen
|
Synthetic non-phosphopeptide derived from Human JunB around the phosphorylation site of Serine 259.
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
Antibody detects endogenous levels of JunB protein around Serine 259.
|
|
Tested applications
(see key)
|
ELISA, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Extracts of Hela cells and breast carcinoma tissue.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
|
|
Relevance
|
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge.
JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, Phosphate buffered saline (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JunB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for JunB antibody images, references, products related to ab31421 and other tools.
|
| |
JunB antibody images:
|
 Immunohistochemistry (Paraffin-embedded sections) - JunB antibody (ab31421)
ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : Un-treated.
Right image : Antibody pre-incubated with synthesized peptide.
|
| |
Western blot - JunB antibody (ab31421)
All lanes : JunB antibody (ab31421) at 1/500 dilution
Lane 1 : Extracts of Hela cells.
Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.
Predicted band size : 35 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
|
| |
References for JunB antibody (ab31421)
|
|
This product has been used in: (two most recent references)
Lemos MC et al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009). PubMed: 19587266
Dobson T et al. Identifying intrinsic and extrinsic determinants that regulate internal initiation of translation mediated by the FMR1 5' leader. BMC Mol Biol 9:89 (2008). WB; Rat. PubMed: 18922172
If you publish research using ab31421 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to JunB
|
| |
JunB antibody - more information
|
Customer reviews (feedback) regarding JunB antibody |
Customer FAQs regarding JunB antibody |
Protocols for JunB antibody |
Price and availability of products related to JunB antibody |
| |
JunB antibody - related products:
|
|
|
| |
JunB antibody - other tools:
|
Contact Abcam with a Technical Enquiry about JunB antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
