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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunB antibody
See all JunB products (9) ...
Description
Rabbit polyclonal to JunB
Specificity
Antibody detects endogenous levels of JunB protein around Serine 259.
Tested applications
ICC/IF, WB, IP, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from Human JunB around the phosphorylation site of Serine 259.
Positive control
Extracts of Hela cells and breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.
IP: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Relevance
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: P17275 Human
- SwissProt: P09450 Mouse
- SwissProt: P24898 Rat
- Unigene: 25292 Human
see all
Anti-JunB antibody images:
Immunohistochemistry (Paraffin-embedded sections) - JunB antibody (ab31421)
ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : Un-treated.
Right image : Antibody pre-incubated with synthesized peptide.
Western blot - JunB antibody (ab31421)
All lanes : Anti-JunB antibody (ab31421) at 1/500 dilution
Lane 1 : Extracts of Hela cells.
Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.
Predicted band size : 35 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-Anti-JunB antibody(ab31421)
ICC/IF image of ab31421 stained Hek293 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-JunB antibody (ab31421)
This product has been referenced in:
- Raffaello Aet al. JunB transcription factor maintains skeletal muscle mass and promotes hypertrophy. J Cell Biol 191:101-13 (2010). WB; Mouse.Read more (PubMed: 20921137) »
- Lemos MCet al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009).Read more (PubMed: 19587266) »
See all 3 publications for this product
Publishing research using ab31421? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"