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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunB antibody
See all JunB products (9) ...
Description
Goat polyclonal to JunB
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Marmoset (common)
Immunogen
Synthetic peptide with sequence C-TLKAENAGLSSTAG, corresponding to internal sequence amino acids 302 - 315 of Human JunB, with a Cysteine residue linker (NP_002220.1.)
C-TLKAENAG LSSTAG
Positive control
Huamn breast cancer lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab87649 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - JunB antibody (ab87649)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Predicted molecular weight: 36 kDa.
Target
Relevance
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 514246 Cow
- Entrez Gene: 484927 Dog
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: Q0VBZ5 Cow
- SwissProt: P17275 Human
see all
Anti-JunB antibody images:
Western blot - JunB antibody (ab87649)
Anti-JunB antibody (ab87649) at 0.3 µg/ml + Human breast cancer cell lysate (in RIPA buffer) at 35 µg/ml
developed using the ECL technique
Predicted band size : 36 kDa
Observed band size : 36 kDa
Primary incubation was 1 hour.
References for Anti-JunB antibody (ab87649)
ab87649 has not yet been referenced specifically in any publications.
Publishing research using ab87649? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"