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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunB (phospho S259) antibody
See all JunB products (9) ...
Description
Rabbit polyclonal to JunB (phospho S259)
Specificity
ab30628 recognises endogenous levels of JunB only when phosphorylated at Serine 259.
Tested applications
WB, ELISA, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human JunB around the phosphorylation site of Serine 259.
Positive control
Breast carcinoma, HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
Target
Relevance
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: P17275 Human
- SwissProt: P09450 Mouse
- SwissProt: P24898 Rat
- Unigene: 25292 Human
see all
Anti-JunB (phospho S259) antibody images:
Western blot - JunB (phospho S259) antibody (ab30628)
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All lanes : Anti-JunB (phospho S259) antibody (ab30628) at 1/500 dilution
Lane 1 : extracts from HeLa cells (5-30ug).
Lane 2 : extracts from HeLa cells (5-30ug), preincubated
with synthesized non phosphopeptide.
Lane 3 : extracts from HeLa cells (5-30ug), preincubated with synthesized
phosphopeptide (negative control).
Predicted band size : 35 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - JunB (phospho S259) antibody (ab30628)
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Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 JunB (Phospho Ser259) Antibody (ab30628). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
References for Anti-JunB (phospho S259) antibody (ab30628)
ab30628 has not yet been referenced specifically in any publications.
Publishing research using ab30628? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"