Overview

  • Product nameAnti-JunD antibody [EPR6520]See all JunD primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6520] to JunD
  • Tested applicationsWB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human JunD.

  • Positive control
    • 293T, Jurkat, HepG2 and HeLa cell lysates; Human skin and kidney tissue; HeLa cells.
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139921.

    A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTranscription factor binding AP-1 sites.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun D antibody
      • jun D proto oncogene antibody
      • Jund antibody
      • JunD FL isoform antibody
      • JUND_HUMAN antibody
      • Transcription factor jun D antibody
      • Transcription factor jun-D antibody
      see all

    Anti-JunD antibody [EPR6520] images

    • All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling JunD with ab134067 at 1/1000 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling JunD with ab134067 at 1/1000 dilution.
    • Immunofluorescent analysis of HeLa cells labelling JunD with ab134067 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-JunD antibody [EPR6520] (ab134067)

    This product has been referenced in:
    • Chen H  et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). WB ; Human . Read more (PubMed: 24107296) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 27 2014

    Application Immunoprecipitation
    Immuno-precipitation step Other - EMSA
    Sample Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
    Specification Renal cancer cell lines - nuclear extract
    Total protein in input 3 µg
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"