Overview

  • Product nameAnti-JunD antibody [EPR6520]
    See all JunD primary antibodies
  • Description
    Rabbit monoclonal [EPR6520] to JunD
  • Tested applicationsWB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human JunD aa 100-200.

  • Positive control
    • 293T, Jurkat, HepG2 and HeLa cell lysates; Human skin and kidney tissue; HeLa cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is available conjugated to DyLight® 488 - see ab139921.

    A 40 μl trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab134067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).

For unpurified, use 1/5000.

IP 1/50.

For unpurified, use 1/30.

ICC/IF 1/300.

For unpurified, use 1/200.

  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionTranscription factor binding AP-1 sites.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun D antibody
      • jun D proto oncogene antibody
      • Jund antibody
      • JunD FL isoform antibody
      • JUND_HUMAN antibody
      • Transcription factor jun D antibody
      • Transcription factor jun-D antibody
      see all

    Anti-JunD antibody [EPR6520] images

    • Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

    • ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-JunD antibody [EPR6520] (ab134067)

    This product has been referenced in:
    • Chen H  et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). WB ; Human . Read more (PubMed: 24107296) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 27 2014

    Application Immunoprecipitation
    Immuno-precipitation step Other - EMSA
    Sample Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
    Specification Renal cancer cell lines - nuclear extract
    Total protein in input 3 µg
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"