Overview

  • Product nameAnti-JunD antibody [EPR6520]
    See all JunD primary antibodies
  • Description
    Rabbit monoclonal [EPR6520] to JunD
  • Tested applicationsWB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human JunD aa 100-200.

  • Positive control
    • 293T, Jurkat, HepG2 and HeLa cell lysates; Human skin and kidney tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab134067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).

For unpurified, use 1/5000.

IP 1/50.

For unpurified, use 1/30.

ICC/IF 1/300.

For unpurified, use 1/200.

  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionTranscription factor binding AP-1 sites.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun D antibody
      • jun D proto oncogene antibody
      • Jund antibody
      • JunD FL isoform antibody
      • JUND_HUMAN antibody
      • Transcription factor jun D antibody
      • Transcription factor jun-D antibody
      see all

    Anti-JunD antibody [EPR6520] images

    • Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

    • ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-JunD antibody [EPR6520] (ab134067)

    This product has been referenced in:
    • Chen H  et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). WB ; Human . Read more (PubMed: 24107296) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 27 2014

    Application Immunoprecipitation
    Immuno-precipitation step Other - EMSA
    Sample Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
    Specification Renal cancer cell lines - nuclear extract
    Total protein in input 3 µg
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"