• Product nameAnti-JunD antibody [EPR6520]
    See all JunD primary antibodies
  • Description
    Rabbit monoclonal [EPR6520] to JunD
  • Tested applicationsWB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human JunD.

  • Positive control
    • 293T, Jurkat, HepG2 and HeLa cell lysates; Human skin and kidney tissue; HeLa cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 - see ab139921.

    A 40 μl trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab134067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTranscription factor binding AP-1 sites.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun D antibody
      • jun D proto oncogene antibody
      • Jund antibody
      • JunD FL isoform antibody
      • JUND_HUMAN antibody
      • Transcription factor jun D antibody
      • Transcription factor jun-D antibody
      see all

    Anti-JunD antibody [EPR6520] images

    • All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 39,43 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling JunD with ab134067 at 1/1000 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling JunD with ab134067 at 1/1000 dilution.
    • Immunofluorescent analysis of HeLa cells labelling JunD with ab134067 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-JunD antibody [EPR6520] (ab134067)

    This product has been referenced in:
    • Chen H  et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). WB ; Human . Read more (PubMed: 24107296) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 27 2014

    Application Immunoprecipitation
    Immuno-precipitation step Other - EMSA
    Sample Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
    Specification Renal cancer cell lines - nuclear extract
    Total protein in input 3 µg

    Abcam user community

    Verified customer

    Submitted Mar 18 2014