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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunD antibody
See all JunD products (8) ...
Description
Rabbit polyclonal to JunD
Specificity
ab28837 recognises JunD.
Tested applications
IHC-P, WB, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.
Positive control
Breast carcinoma, extracts from Hela cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
IP: Use at an assay dependent dilution.
Target
Relevance
JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
Anti-JunD antibody images:
Western blot - JunD antibody (ab28837)
Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)
Lane 1 : extracts from Hela cells.
Lane 2 : extracts from Hela cells.
Predicted band size : 35 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837)
Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).
References for Anti-JunD antibody (ab28837)
This product has been referenced in:
- Tili Eet al. Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31:1561-6 (2010). WB; Human.Read more (PubMed: 20622002) »
- Lemos MCet al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009).Read more (PubMed: 19587266) »
See all 2 publications for this product
Publishing research using ab28837? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"