You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-JunD antibody (ab28837)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-JunD antibody
See all JunD products (8) ...

Description

Rabbit polyclonal to JunD

Specificity

ab28837 recognises JunD.

Tested applications

IHC-P, WB, ELISA, IPmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.

Positive control

Breast carcinoma, extracts from Hela cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - JunD antibody (ab28837)Western blot - JunD antibody (ab28837) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837)Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

Target

Relevance

JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.

Cellular localization

Nuclear

Alternative names

  • Activator protein 1 antibody
  • AP 1 antibody
  • AP1 antibody
  • Jun D antibody
  • jun D proto oncogene antibody
  • JunD FL isoform antibody
  • Transcription factor jun D antibody
see all

Anti-JunD antibody images:

  Western blot - JunD antibody (ab28837)

Western blot - JunD antibody (ab28837)

Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)

Lane 1 : extracts from Hela cells.
Lane 2 : extracts from Hela cells.


Predicted band size : 35 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837)

Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837)

Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).

References for Anti-JunD antibody (ab28837)

This product has been referenced in:

  • Tili Eet al. Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31:1561-6 (2010). WB; Human.Read more (PubMed: 20622002) »
  • Lemos MCet al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009).Read more (PubMed: 19587266) »

See all 2 publications for this product

Publishing research using ab28837? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"