Overview

  • Product nameAnti-JunD antibodySee all JunD primary antibodies ...
  • Description
    Rabbit polyclonal to JunD
  • Specificityab28837 recognises JunD.
  • Tested applicationsIHC-P, WB, ELISA, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.

  • Positive control
    • Breast carcinoma, extracts from Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/20000.
IP Use at an assay dependent dilution.

Target

Anti-JunD antibody images

  • Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
    Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)

    Lane 1 : extracts from Hela cells.
    Lane 2 : extracts from Hela cells.


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).

References for Anti-JunD antibody (ab28837)

This product has been referenced in:
  • Tili E  et al. Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31:1561-6 (2010). WB ; Human . Read more (PubMed: 20622002) »
  • Lemos MC  et al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009). Read more (PubMed: 19587266) »

See all 2 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - EMSA
Sample Human Cell lysate - nuclear (renal cancer cells)
Specification renal cancer cells
Total protein in input 3 µg
Username

Abcam user community

Verified customer

Submitted Mar 14 2014

Thank you for your inquiry. Firstly, this antibody is not phospho-specific. It will recognize the total protein. The immunogen is a synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255. We g...

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Thank you for contacting us. The antibody was raised against a sequence around Serine 255 of human JunD. It is not a phospho antibody, though, and will bind the protein only if it is NOT phosphorylated at S255. Regarding the 2 isoforms: ...

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Thank you for contacting us. All our product are covered by Abpromise guarantee which states we replace or fully refund products not performing as stated on the datasheet when reported within 6 months of delivery. Please click the follow...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"