Overview
- Product nameAnti-JunD antibodySee all JunD primary antibodies ...
- DescriptionRabbit polyclonal to JunD
- Specificityab28837 recognises JunD.
- Tested applicationsIHC-P, WB, ELISA, IP more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.
- Positive controlBreast carcinoma, extracts from Hela cells.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab28837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 35 kDa. |
| ELISA | ELISA: 1/20000. |
| IP | IP: Use at an assay dependent dilution. |
Target
- FunctionTranscription factor binding AP-1 sites.
- Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- Unigene: 2780 Human
- Unigene: 419851 Mouse
Target information above from: UniProt accession
P17535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Activator protein 1 antibodyAP 1 antibodyAP1 antibody
- Jun D antibodyjun D proto oncogene antibodyJund antibodyJunD FL isoform antibodyJUND_HUMAN antibodyTranscription factor jun D antibodyTranscription factor jun-D antibody
see all
Anti-JunD antibody images
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Western blot - JunD antibody (ab28837)Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)
Lane 1 : extracts from Hela cells.
Lane 2 : extracts from Hela cells.
Predicted band size : 35 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - JunD antibody (ab28837)Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).
References for Anti-JunD antibody (ab28837)
This product has been referenced in:
- Tili E et al. Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31:1561-6 (2010). WB ; Human . Read more (PubMed: 20622002) »
- Lemos MC et al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009). Read more (PubMed: 19587266) »
