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Product Name
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JunD antibody
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See all JunD antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JunD
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Immunogen
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Fusion protein.
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Reacts with
(species key)
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Hu, Bird
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Specificity
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Reacts specifically with 48 kDa protein derived from a transfected CEF cell line.
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Tested applications
(see key)
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EMSA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions EMSA: Use at an assay dependent dilution. WB: 1/200 - 1/5000. Predicted molecular weight: 35 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Transcription factors Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: Whole Serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JunD antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for JunD antibody (ab425)
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This product has been used in:
Bobrovnikova-Marjon EV et al. Expression of angiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 is highly responsive to ambient glutamine availability: role of nuclear factor-kappaB and activating protein-1. Cancer Res 64:4858-69 (2004). PubMed: 15256456
If you publish research using ab425 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to JunD
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JunD antibody - more information
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Customer reviews (feedback) regarding JunD antibody |
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Price and availability of products related to JunD antibody |
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JunD antibody - related products:
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Contact Abcam with a Technical Enquiry about JunD antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"