JunD antibody (ab425)

Product Name 

JunD antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JunD

Immunogen 

Fusion protein.

Reacts with 
(species key)

Hu, Bird

Specificity 

Reacts specifically with 48 kDa protein derived from a transfected CEF cell line.

Tested applications 
(see key)

EMSA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
EMSA: Use at an assay dependent dilution.
WB: 1/200 - 1/5000. Predicted molecular weight: 35 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole Serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for JunD antibody (ab425)

  This product has been used in:

Bobrovnikova-Marjon EV et al. Expression of angiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 is highly responsive to ambient glutamine availability: role of nuclear factor-kappaB and activating protein-1. Cancer Res 64:4858-69 (2004). PubMed: 15256456


If you publish research using ab425 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding JunD antibody

Customer FAQs regarding JunD antibody

Protocols for JunD antibody

Price and availability of products related to JunD antibody

JunD antibody - related products: