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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunD antibody
See all JunD products (8) ...
Description
Rabbit polyclonal to JunD
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken
Immunogen
Synthetic peptide: GCQLLPQHQVPAY, corresponding to amino acids 329-341 of Mouse JunD.
GCQLLPQHQV PAY
Positive control
Mouse cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 37-41 kDa (predicted molecular weight: 41.6 kDa) representing JunD from both un-induced and induced murine NIH 3T3 cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24518 Rat
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- SwissProt: P52909 Rat
References for Anti-JunD antibody (ab5799)
This product has been referenced in:
- Govindarajan Bet al. Overexpression of Akt converts radial growth melanoma to vertical growth melanoma. J Clin Invest 117:719-29 (2007). WB; Human.Read more (PubMed: 17318262) »
See 1 publication for this product
Publishing research using ab5799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"