JunD antibody (ab5799)

Product Name 

JunD antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JunD

Immunogen 

Synthetic peptide: GCQLLPQHQVPAY, corresponding to amino acids 329-341 of Mouse JunD.
BLAST 'GCQLLPQHQVPAY' with or

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 37-41 kDa (predicted molecular weight: 41.6 kDa) representing JunD from both un-induced and induced murine NIH 3T3 cells.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse cells.

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for JunD antibody (ab5799)

  This product has been used in:

Govindarajan B et al. Overexpression of Akt converts radial growth melanoma to vertical growth melanoma. J Clin Invest 117:719-29 (2007). WB; Human. PubMed: 17318262

If you publish research using ab5799 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding JunD antibody

Customer FAQs regarding JunD antibody

Protocols for JunD antibody

Price and availability of products related to JunD antibody

JunD antibody - related products: