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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunD antibody
See all JunD products (8) ...
Description
Rabbit polyclonal to JunD
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human JunD.
(Peptide available as ab74426.)
Positive control
This antibody gave a positive signal in the following Human Lysates: HeLa Nuclear, Jurkat Nuclear, HEK293 Whole Cell, HepG2 Nuclear
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
Target
Relevance
JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24518 Rat
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- SwissProt: P52909 Rat
Anti-JunD antibody images:
Western blot - JunD antibody (ab67453)
All lanes : Anti-JunD antibody (ab67453) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 130 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
Immunocytochemistry/ Immunofluorescence - JunD antibody (ab67453)
ICC/IF image of ab67453 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67453, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
References for Anti-JunD antibody (ab67453)
ab67453 has not yet been referenced specifically in any publications.
Publishing research using ab67453? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"