JunD (phospho S255) antibody (ab30631)

Product Name 

JunD (phospho S255) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JunD (phospho S255)

Immunogen 

Synthetic phosphopeptide derived from Human JunD around the phosphorylation site of Serine 255.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Antibody detects endogenous levels of JunD only when phosphorylated at Serine 255.

Tested applications 
(see key)

ELISA, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 35 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cells.

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Affinity purified fom rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed using chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for JunD (phospho S255) antibody images, references, products related to ab30631 and other tools.

JunD (phospho S255) antibody images:

References for JunD (phospho S255) antibody (ab30631)

  This product has been used in:

Govindarajan B et al. Overexpression of Akt converts radial growth melanoma to vertical growth melanoma. J Clin Invest 117:719-29 (2007). WB; Human. PubMed: 17318262

If you publish research using ab30631 please let us know so that we can cite the reference on this datasheet.

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