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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-JunD (phospho S255) antibody
See all JunD products (8) ...
Description
Rabbit polyclonal to JunD (phospho S255)
Specificity
Antibody detects endogenous levels of JunD only when phosphorylated at Serine 255.
Tested applications
WB, ELISA, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from Human JunD around the phosphorylation site of Serine 255.
Positive control
293 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified fom rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed using chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
Target
Relevance
JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
Cellular localization
Nuclear
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24518 Rat
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- SwissProt: P52909 Rat
Anti-JunD (phospho S255) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - JunD (phospho S255) antibody (ab30631)
ab30631 staining human JunD in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Left image un-treated.
Right image pre-incubated with synthetic phosphopeptide.
Western blot - JunD (phospho S255) antibody (ab30631)
All lanes : Anti-JunD (phospho S255) antibody (ab30631)
Lane 1 : Un-treated 293 cells.
Lane 2 : 293 cells treated with forskolin.
Predicted band size : 35 kDa
References for Anti-JunD (phospho S255) antibody (ab30631)
This product has been referenced in:
- Govindarajan Bet al. Overexpression of Akt converts radial growth melanoma to vertical growth melanoma. J Clin Invest 117:719-29 (2007). WB; Human.Read more (PubMed: 17318262) »
See 1 publication for this product
Publishing research using ab30631? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"