![Flow Cytometry - Junctional Adhesion Molecule 1 antibody [H202-106] (ab36448) image](#Junctional-Adhesion-Molecule-1-Primary-antibodies-ab36448-1.gif)
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Product name
Anti-Junctional Adhesion Molecule 1 antibody [H202-106]
See all Junctional Adhesion Molecule 1 products (10) ...
Description
Rat monoclonal [H202-106] to Junctional Adhesion Molecule 1
Specificity
ab36448 recognises CD321, which is also known as junctional adhesion molecule 1 (JAM-1).
Tested applications
Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Mouse
Immunogen
MTE1/MTE2 stromal cell lines (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
H202-106
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell junction molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Cardiovascular >> Blood >> Platelets
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Flow Cytometry - Junctional Adhesion Molecule 1 antibody [H202-106] (ab36448)
![Flow Cytometry - Junctional Adhesion Molecule 1 antibody [H202-106] (ab36448) image](/ps/datasheet/images/36/ab36448/Junctional-Adhesion-Molecule-1-Primary-antibodies-ab36448-1.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab36448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
Flow Cyt
IHC-Fr
IP
Application notes
Flow Cyt: 1/100 - 1/200. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
Target information above from: UniProt accessionQ9Y624
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD321 antibody
- CD 321 antibody
- CD321 antibody
see all
Database links
- Entrez Gene: 16456 Mouse
- SwissProt: O88792 Mouse
- Unigene: 294882 Mouse
Anti-Junctional Adhesion Molecule 1 antibody [H202-106] images:
Flow Cytometry - Junctional Adhesion Molecule 1 antibody [H202-106] (ab36448)
ab36448 staining Junctional Adhesion Molecule 1 in mouse peripheral blood platelets by Flow cytometry.
References for Anti-Junctional Adhesion Molecule 1 antibody [H202-106] (ab36448)
This product has been referenced in:
- Saha A et al. The blood-brain barrier is disrupted in a mouse model of infantile neuronal ceroid lipofuscinosis: amelioration by resveratrol. Hum Mol Genet : (2012). WB; Mouse.Read more (PubMed: 22331300) »
- Aurrand-Lions M et al. Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood 98:3699-707 (2001).Read more (PubMed: 11739175) »
See all 2 publications for this product
Publishing research using ab36448? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"