Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab91501)
Key features and details
- Goat polyclonal to Junctional Adhesion Molecule 1/JAM-A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Junctional Adhesion Molecule 1/JAM-A antibody
See all Junctional Adhesion Molecule 1/JAM-A primary antibodies -
Description
Goat polyclonal to Junctional Adhesion Molecule 1/JAM-A -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human Junctional Adhesion Molecule 1/JAM-A aa 282-295 (C terminal).
Sequence:C-QPSARSEGEFKQTS
Database link: NP_058642.1 -
Positive control
- Human peripheral bood mononucleocyte lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab91501 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 50848 Human
- Entrez Gene: 16456 Mouse
- Entrez Gene: 116479 Rat
- Omim: 605721 Human
- SwissProt: Q9Y624 Human
- SwissProt: O88792 Mouse
- SwissProt: Q9JHY1 Rat
- Unigene: 517293 Human
see all -
Alternative names
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
see all
Images
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Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab91501) at 0.3 µg/ml + Human Peripheral Blood Mononucleocyte lysate at 35 µg
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Datasheets and documents
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Datasheet download
References (0)
ab91501 has not yet been referenced specifically in any publications.