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Anti-Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643)

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Overview

Product name

Anti-Junctional Adhesion Molecule 2 antibody [CRAM-19 H36]
See all Junctional Adhesion Molecule 2 products (8) ...

Description

Rat monoclonal [CRAM-19 H36] to Junctional Adhesion Molecule 2

Specificity

ab23643 recognises junctional adhesion molecule-2 (JAM-2).

Tested applications

Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant soluble full length JAM-2 protein (Mouse).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

CRAM-19 H36

Myeloma

Sp2/0

Isotype

IgG2a

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial

  • Flow Cytometry - Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643)Flow Cytometry - Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab23643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/50 - 1/100.
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May play a role in the processes of lymphocyte homing to secondary lymphoid organs.

Tissue specificity

Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.

Sequence similarities

Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Cellular localization

Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.

Target information above from: UniProt accessionP57087 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • C21orf43 antibody
  • CAM antibody
  • CD 322 antibody
  • CD322 antibody
  • CD322 antigen antibody
  • JAM 2 antibody
  • JAM B antibody
  • JAM IT/VE JAM antibody
  • JAM, vascular endothelial antibody
  • JAM-2 antibody
  • JAM-B antibody
  • JAM2 antibody
  • JAM2_HUMAN antibody
  • JAMB antibody
  • JAMC antibody
  • Jcam2 antibody
  • Junction adhesion molecule 2 antibody
  • Junction adhesion molecule B antibody
  • Junction adhesion molecule C antibody
  • Junctional adhesion molecule 2 antibody
  • Junctional adhesion molecule B antibody
  • PRO 245 antibody
  • PRO245 antibody
  • Vascular endothelial junction associated molecule antibody
  • Vascular endothelial junction-associated molecule antibody
  • VE JAM antibody
  • VE-JAM antibody
  • VEJAM antibody
see all

Database links

Anti-Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] images:

  Flow Cytometry - Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643)

Flow Cytometry - Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643)

ab23643 staining human H460 lung cancer cells by flow cytometry.  Cells were harvested in cell dissociation buffer and resuspended in an enzyme free buffer.  Cells were fixed with formaldehyde and incubated with ab26343 (diluted 1/100) for 30 minutes at 4°C.  A phycoerythrin conjugated goat anti-rat IgG anitbody was used as the secondary.  The flow cytometry plot shows JAM2 expression on H460 (green) and H460SM (pink) cell lines

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Junctional Adhesion Molecule 2 antibody [CRAM-19 H36] (ab23643)

ab23643 has not yet been referenced specifically in any publications.

Publishing research using ab23643? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"