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Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)

Q&A (2)

Overview

  • Product nameJurkat (Human T cell lymphoblast-like cell line) Nuclear LysateSee all Jurkat lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

    • References for Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)

    ab14844 has not yet been referenced specifically in any publications.

    Publishing research using ab14844 ? Please let us know so that we can cite the reference in this datasheet

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    Hi, I wanted to know which antibodies the cell lysate was probed with to confirm nuclear markers and exclude the presence of cytoplasmic markers. Thank you.

    Thank you for your enquiry. We probed for nuclear markers, mainly transcription factors such as ATF-2, E12/47, GATA-2, NF-yB, Tal-1, USF, SP1, AML-1. Unfortunately there is no data available showing the absence of cytoplasmic markers. I hope this in...

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    I'd like to use this nuclear lysate for my PPAR-gamma, delta Western-blot as a positive control. Do I have to boil it up or use any kind of pre-treatment with this lysate or it is ready to load. Typically how many microgramms do you suggest to load for a ...

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    Thank you for your enquiry and your interest in our products. Ab14844 is Jurkat nuclear lysate and you need to dilute it in a standard Western blot sample buffer. It may be worth boiling the sample for 1 or 2 minutes before loading. Usually for...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"