Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)

Overview

  • Product nameJurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateSee all Jurkat lysates ...
  • General notesCell line: Jurkat, clone E6-1 (Human acute T cell leukemia) Growth media: RPMI & 10% NCS (Newborn calf serum)
  • Tested applicationsWBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notes Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodiumchloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas
  • BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Applications

Our Abpromise guarantee covers the use of ab7899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Jurkat cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

References for Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)

This product has been referenced in:
  • Berra-Romani R  et al. Ca2+ handling is altered when arterial myocytes progress from a contractile to a proliferative phenotype in culture. Am J Physiol Cell Physiol 295:C779-90 (2008). Read more (PubMed: 18596214) »

See 1 Publication for this product

Product Wall

Thank you for sending this data to me.

While reviewing this slide, I noted that your bands are white. Oversaturation of secondary antibodies can lead to masked signal or false positive. In this case, the secondary antibody which you are us...

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I was not able to view the attachment included. Would you be able to re-send this to me as a jpeg, ppt or png file? Also could you let me know some of the details of the experiement (amount of positive control ...

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Thank you for confirming these details and for your cooperation. I am sorry that you are continuing to have difficulties when using these products. As requested, I have issued a free of charge replacement of ab73229 PDGF BB Protein and have arrange ...

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According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.
...

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I'm sorry to hear you are experiencing problems with our Jurkat lysate ab7899 and have tried to look at the [ a competitor] antibodies to see if they were the same as ab12034 and ab12035. In fact they are not the same antibodies but the same clones, a...

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Please let me know what extra information you require. This product is a Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate which we sell in units of 100µg in 1 x SDS sample buffer containing 5% b-mercaptoethanol. There is further infor...

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Thank you for your email and I'm sorry to hear about the difficulty you are experiencing with ab4788. I apologize for the shortage that you received and can certainly send you a replacement vial free of charge. Can you please tell me the batch numb...

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Yes, Jurkat cell lysate would be a good positive control for active caspase 9. Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"