Jurkat nuclear extract lysate (ab14844)

Overview

  • Product name
    Jurkat nuclear extract lysate
    See all Jurkat lysates
  • General notes
    Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

References

ab14844 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. We probed for nuclear markers, mainly transcription factors such as ATF-2, E12/47, GATA-2, NF-yB, Tal-1, USF, SP1, AML-1. Unfortunately there is no data available showing the absence of cytoplasmic markers. I hope this...

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Thank you for your enquiry and your interest in our products. Ab14844 is Jurkat nuclear lysate and you need to dilute it in a standard Western blot sample buffer. It may be worth boiling the sample for 1 or 2 minutes before loading. Usually for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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