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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab10101? Please let us know so that we can cite the reference in this datasheet
ab10101 has been referenced in 1 publications.
- Chen ZL et al. Proteolytic fragments of laminin promote excitotoxic neurodegeneration by up-regulation of the KA1 subunit of the kainate receptor. J Cell Biol 183:1299-313 (2008). IHC-FoFr; Mouse. PubMed: 19114596
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - KA1 antibody (ab10101)
ICC/IF image of ab10101 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10101, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - KA1 antibody (ab10101)
Anti-KA1 antibody (ab10101) at 1/1000 dilution + Mouse lung
Predicted band size : 107 kDa
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