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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Overview
Product name
Anti-KA1 antibody
See all KA1 products (4) ...
Description
Rabbit polyclonal to KA1
Tested applications
WB, ICC/IF, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 60-73 of Human KA1.
Positive control
Mouse lung lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Sucrose
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
ICC/IF: Use a concentration of 5 µg/ml
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19114596)
Target
Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Target information above from: UniProt accessionQ16099
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ionotropic kainate 4 antibody
- EAA 1 antibody
- EAA1 antibody
see all
Database links
- Entrez Gene: 2900 Human
- Entrez Gene: 110637 Mouse
- Entrez Gene: 24406 Rat
- Omim: 600282 Human
- SwissProt: Q16099 Human
- SwissProt: Q8BMF5 Mouse
- SwissProt: Q01812 Rat
- Unigene: 222901 Human
see all
Anti-KA1 antibody images:
Immunocytochemistry/ Immunofluorescence - KA1 antibody (ab10101)
ICC/IF image of ab10101 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10101, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - KA1 antibody (ab10101)
Anti-KA1 antibody (ab10101) at 1/1000 dilution + Mouse lung
Predicted band size : 107 kDa
References for Anti-KA1 antibody (ab10101)
This product has been referenced in:
- Chen ZLet al. Proteolytic fragments of laminin promote excitotoxic neurodegeneration by up-regulation of the KA1 subunit of the kainate receptor. J Cell Biol 183:1299-313 (2008). IHC-FoFr; Mouse.Read more (PubMed: 19114596) »
See 1 publication for this product
Publishing research using ab10101? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"