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Western blot - KA1 antibody (ab10101)
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Product Name
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KA1 antibody
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See all KA1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KA1
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Immunogen
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Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 60-73 of Human KA1.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-FoFr: Use at an assay dependent dilution (PMID 19114596).
WB: 1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 114.7 kDa).
ELISA: positive result against immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse lung lysate.
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Cellular localization
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Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
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Research areas
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Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
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Relevance
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KA1 is a high affinity subunit of kainate-type ionotropic glutamate receptors which are critical regulators of network activity that act by modifying neuronal excitability, directly and indirectly, through GABAergic interneurons. Kainate receptors on GABA-containing interneurons enhance GABA release and thereby downregulate glutamatergic signaling. KARs have been implicated in numerous psychiatric disorders. Case control studies show significant association of KA1 with both schizophrenia and bipolar disorder.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, Sucrose
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KA1 antibody images, references, products related to ab10101 and other tools.
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KA1 antibody images:
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 Western blot - KA1 antibody (ab10101)
Predicted band size : 114.7 kDa
Western Blot for ab10101 [A] Duplicate run of anti-KA1 (10µg/ml) on whole rat brain homogenate. [B] Single run of anti-KA1 binding in the presence of immunising peptide (ab14283).Observe absence of 140 kDa band (the mature form of the KA1 receptor subunit) [C] Secondary control run. Lane incubated with secondary antibody goat anti-rabbit (HRP alone)
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References for KA1 antibody (ab10101)
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This product has been used in:
Chen ZL et al. Proteolytic fragments of laminin promote excitotoxic neurodegeneration by up-regulation of the KA1 subunit of the kainate receptor. J Cell Biol 183:1299-313 (2008). IHC-FoFr; Mouse. PubMed: 19114596
If you publish research using ab10101 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KA1
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KA1 antibody - more information
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Customer reviews (feedback) regarding KA1 antibody |
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Price and availability of products related to KA1 antibody |
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KA1 antibody - related products:
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KA1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about KA1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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