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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Overview
Product name
Anti-KA1 antibody
See all KA1 products (4) ...
Description
Rabbit polyclonal to KA1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A 14 amino acid peptide near the amino terminus of human KA1.
Positive control
Rat brain lysate and human brain tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa).
IHC-P: Use at an assay dependent dilution.
Target
Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Target information above from: UniProt accessionQ16099
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ionotropic kainate 4 antibody
- EAA 1 antibody
- EAA1 antibody
see all
Database links
- Entrez Gene: 2900 Human
- Entrez Gene: 110637 Mouse
- Entrez Gene: 24406 Rat
- Omim: 600282 Human
- SwissProt: Q16099 Human
- SwissProt: Q8BMF5 Mouse
- SwissProt: Q01812 Rat
- Unigene: 222901 Human
see all
Anti-KA1 antibody images:
Western blot - KA1 antibody (ab67404)
Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
Lane 1 : rat brain lysate
Lane 2 : rat brain lysate
Lane 3 : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 107 kDa
Observed band size : <97 kDa (why is the actual band size different from the predicted?)
Additional bands at : 108 kDa. We are unsure as to the identity of these extra bands.
N.B. Despite its predicted molecular weight, KA1 often migrates at a lower molecular weight in SDS-PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KA1 antibody (ab67404)
ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.
Immunocytochemistry/ Immunofluorescence - Anti-KA1 antibody (ab67404)
ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KA1 antibody (ab67404)
ab67404 has not yet been referenced specifically in any publications.
Publishing research using ab67404? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"