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Read our guarantee »Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Anti-KA1 antibody
See all KA1 products (4) ...
Rabbit polyclonal to KA1
WB, IHC-Pmore details
Reacts with
Mouse, Rat, Human
A 14 amino acid peptide near the amino terminus of human KA1.
Rat brain lysate and human brain tissue.
Liquid
Store at +4°C.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> AMPA / Kainate
Our Abpromise guarantee covers the use of ab67404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa).
IHC-P: Use at an assay dependent dilution.
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Target information above from: UniProt accessionQ16099
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - KA1 antibody (ab67404)

Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
Lane 1 : rat brain lysate
Lane 2 : rat brain lysate
Lane 3 : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 107 kDa
Observed band size : <97 kDa (why is the actual band size different from the predicted?)
Additional bands at : 108 kDa. We are unsure as to the identity of these extra bands.
N.B. Despite its predicted molecular weight, KA1 often migrates at a lower molecular weight in SDS-PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KA1 antibody (ab67404)

ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.
Immunocytochemistry/ Immunofluorescence - Anti-KA1 antibody (ab67404)

ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab67404 has not yet been referenced specifically in any publications.
Publishing research using ab67404? Please let us know so that we can cite the reference in this datasheet
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Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
Lane 1 : rat brain lysate
Lane 2 : rat brain lysate
Lane 3 : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 107 kDa
Observed band size : <97 kDa (why is the actual band size different from the predicted?)
Additional bands at : 108 kDa. We are unsure as to the identity of these extra bands.
N.B. Despite its predicted molecular weight, KA1 often migrates at a lower molecular weight in SDS-PAGE.

ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.

ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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