Anti-KALRN antibody (ab52012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KALRN antibody (ab52012)

ab52012 staining KALRN in adult brain tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step performed using citrate buffer. Tissues were then blocked with 15% serum for 45 minutes at 20ºC followed by incubation with the primary antibody, at a 1/250 dilution, for 24 hours at 4ºC. A Biotin-conjugated rabbit anti-goat IgG was used as secondary antibody at a 1/250 dilution.

Upper image: Un-treated.

Lower image: Treated with peptide to KALRN.

Counterstained with Hämalaun.

Image kindly supplied by Dr Janine Magg through Abreview

Western blot - KALRN antibody (ab52012)

All lanes : Anti-KALRN antibody (ab52012) at 1/500 dilution

Lane 1 : Whole tissue lysate prepared from mouse brain, treated with peptide.
Lane 2 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN (with FLAG-tag) treated with peptide.
Lane 3 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag, detected with anti-FLAG antibody.
Lane 4 : Whole tissue lysate prepared from mouse brain
Lane 5 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag.

Lysates/proteins at 30 µg per lane.

Secondary
Donkey anti-goat IgG conjugated to HRP at 1/10000 dilution
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 192 kDa
Observed band size : 192 kDa


Exposure time : 1 minute

8% gel run under denaturing conditions.

Image kindly supplied by Dr Janine Magg through Abreview

Western blot - Anti-KALRN antibody (ab52012)

Anti-KALRN antibody (ab52012) at 0.5 µg/ml + Mouse Brain lysate in RIPA buffer at 35 µg
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 192 kDa


Primary incubation was 1 hour.