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Anti-KALRN antibody (ab52012)

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Overview

Product name

Anti-KALRN antibody
See all KALRN products (3) ...

Description

Goat polyclonal to KALRN

Specificity

This antibody is expected to recognize isoform 2 (NP_003938.1).

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide: C-RWHLGPGDPFSTYV, corresponding to C terminal amino acids 1650-1663 of Human KALRN

C-RWHLGPGD PFSTYV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Mouse Brain lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

  • Western blot - KALRN antibody (ab52012)Western blot - KALRN antibody (ab52012) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KALRN antibody (ab52012)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KALRN antibody (ab52012) image (enlarge)

  • Western blot - KALRN antibody (ab52012)Western blot - KALRN antibody (ab52012) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: antibody detection limit dilution 1:4,000.

WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.

Tissue specificity

Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.

Involvement in disease

Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 CRAL-TRIO domain.
Contains 2 DH (DBL-homology) domains.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 PH domains.
Contains 1 protein kinase domain.
Contains 2 SH3 domains.
Contains 5 spectrin repeats.

Domain

The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity.

Post-translational
modifications

Autophosphorylated.

Cellular localization

Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.

Target information above from: UniProt accessionO60229 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Duo antibody
  • FLJ16443 antibody
  • HAPIP antibody
  • Huntingtin associated protein interacting protein (duo) antibody
  • Huntingtin-associated protein-interacting protein antibody
  • Kalirin (isoform 2) antibody
  • Kalirin antibody
  • KALRN antibody
  • KALRN_HUMAN antibody
  • Protein Duo antibody
  • RhoGEF kinase antibody
  • Serine/threonine kinase with Dbl and pleckstrin homology domains antibody
  • Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain antibody
  • TRAD antibody
see all

Database links

see all

Anti-KALRN antibody images:

  Western blot - KALRN antibody (ab52012)

Western blot - KALRN antibody (ab52012)

Anti-KALRN antibody (ab52012) at 2 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).

Predicted band size : 192 kDa
Observed band size : 192 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KALRN antibody (ab52012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KALRN antibody (ab52012)

ab52012 staining KALRN in adult brain tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step performed using citrate buffer. Tissues were then blocked with 15% serum for 45 minutes at 20°C followed by incubation with the primary antibody, at a 1/250 dilution, for 24 hours at 4°C. A Biotin-conjugated rabbit anti-goat IgG was used as secondary antibody at a 1/250 dilution.

Upper image: Un-treated.

Lower image: Treated with peptide to KALRN.

Counterstained with Hämalaun.

Image kindly supplied by Dr Janine Magg through Abreview

See Abreview

  Western blot - KALRN antibody (ab52012)

Western blot - KALRN antibody (ab52012)

All lanes : Anti-KALRN antibody (ab52012) at 1/500 dilution

Lane 1 : Whole tissue lysate prepared from mouse brain, treated with peptide.
Lane 2 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN (with FLAG-tag) treated with peptide.
Lane 3 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag, detected with anti-FLAG antibody.
Lane 4 : Whole tissue lysate prepared from mouse brain
Lane 5 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag.

Lysates/proteins at 30 µg per lane.

Secondary
Donkey anti-goat IgG conjugated to HRP at 1/10000 dilution
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 192 kDa
Observed band size : 192 kDa


Exposure time : 1 minute

8% gel run under denaturing conditions.

Image kindly supplied by Dr Janine Magg through Abreview

See Abreview

References for Anti-KALRN antibody (ab52012)

ab52012 has not yet been referenced specifically in any publications.

Publishing research using ab52012? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"