KAP1 antibody [20C1] (ab22553)

Product Name 

KAP1 antibody [20C1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [20C1] to KAP1

Immunogen 

Recombinant fragment, corresponding to amino acids 60-383 of Human KAP1.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

EMSA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
EMSA: Use at an assay dependent dilution (PMID 18287239).
ICC/IF: Use at a concentration of 2 - 4 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 88.5 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Gamma irradiated HeLa cell lysate (WB), and cells (ICC).

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA

Relevance 

The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1).
KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

20C1

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for KAP1 antibody [20C1] (ab22553)

  This product has been used in: (two most recent references)

Noon AT et al. 53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Nat Cell Biol 12:177-84 (2010). PubMed: 20081839

Crawford NP et al. The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284:28660-73 (2009). WB, ICC/IF; Human. PubMed: 19710015

See all 3 publication references for this product.


If you publish research using ab22553 please let us know so that we can cite the reference on this datasheet.

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