|
|
|
|
Western blot - KAP1 antibody (ab10483)
(enlarge)
|
|
|
|
|
Product Name
|
KAP1 antibody - ChIP Grade
|
| |
See all KAP1 antibodies (14)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to KAP1 - ChIP Grade
|
|
Immunogen
|
Immunogen was a synthetic peptide, which represented a portion of human tripartite motif-containing 28 (KAP1) encoded within exon 1 (LocusLink ID 10155).
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ChIP, ChIP/Chip, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 17542650).
ChIP/Chip: Use at an assay dependent dilution (PMID 17542650).
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/500 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Whole cell lysate from normal 293T cells or 293T cells transiently transfected with a human KAP1 construct.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA
|
|
Relevance
|
The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1).
KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: Tris-citrate/phosphate. pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Antibodies were affinity purified using the peptide immobilized on solid support.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAP1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for KAP1 antibody - ChIP Grade images, references, products related to ab10483 and other tools.
|
| |
KAP1 antibody - ChIP Grade images:
|
 Western blot - KAP1 antibody (ab10483)
Predicted band size : 100 kDa
Detection of Human KAP1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 ug for E, 10 ug for T) from normal 293T cells (E) or 293T cells transiently transfected with a human KAP1 construct (T). B. Whole cell lysate (0.5 mg) from normal 293T cells. Antibodies: ab10483 used at the indicated concentration for WB (A) or 2 ug/0.5 mg lysate for IP (B). Immunoprecipitated KAP1 was detected using a KAP1 antibody from another source. Detection: Chemiluminescence with a 1 minute exposure.
|
| |
References for KAP1 antibody - ChIP Grade (ab10483)
|
|
This product has been used in: (two most recent references)
Hu G et al. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev 23:837-48 (2009). ChIP; Mouse. PubMed: 19339689
Rambaud J et al. TIF1beta/KAP-1 is a coactivator of the orphan nuclear receptor NGFI-B/Nur77. J Biol Chem 284:14147-56 (2009). WB; Human. PubMed: 19321449
See all 3 publication references for this product.
If you publish research using ab10483 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to KAP1
|
| |
KAP1 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding KAP1 antibody - ChIP Grade |
Customer FAQs regarding KAP1 antibody - ChIP Grade |
Protocols for KAP1 antibody - ChIP Grade |
Price and availability of products related to KAP1 antibody - ChIP Grade |
| |
KAP1 antibody - ChIP Grade - related products:
|
|
|
| |
KAP1 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about KAP1 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
