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Synthetic peptide: SSQELSGGPGDGP, corresponding to amino acids 741-753 of KAP 1.
Our Abpromise guarantee covers the use of ab3831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 21170338|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 16880268|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human KAP1 peptide (ab22963).|
|IP||Use at an assay dependent concentration. PubMed: 16880268|