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Western blot - TIF1 beta antibody (ab43760)
(enlarge)
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Product Name
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KAP1 antibody
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See all KAP1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to KAP1
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Immunogen
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Synthetic peptide: PAPMAPPRAP GPLSKQGSGS SQPMEVQEGY GFGSGDDPYS SAEPHVSGVK RSRSGEGEVS GLMRKVPRVS LERLDLDLTA DSQPPVFKVF PGSTTEDYNL , corresponding to amino acids 420-520 of Human KAP1
BLAST 'PAPMAPPRAP GPLSKQGSGS SQPMEVQEGY GFGSGDDPYS SAEPHVSGVK RSRSGEGEVS GLMRKVPRVS LERLDLDLTA DSQPPVFKVF PGSTTEDYNL ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 89 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA
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Relevance
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The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1).
KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, Whole serum Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KAP1 antibody images, references, products related to ab43760 and other tools.
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KAP1 antibody images:
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 Western blot - TIF1 beta antibody (ab43760)
All lanes : KAP1 antibody (ab43760) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100ng of GST fusion protein of an irrelevant antigen at 20 µg
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein)
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution
Predicted band size : 89 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note: the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
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Search PubMed (MEDLINE) for references to KAP1
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KAP1 antibody - more information
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Customer reviews (feedback) regarding KAP1 antibody |
Customer FAQs regarding KAP1 antibody |
Protocols for KAP1 antibody |
Price and availability of products related to KAP1 antibody |
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KAP1 antibody - related products:
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KAP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about KAP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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