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Anti-KAT1 / HAT1 antibody (ab12163)

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Overview

Product name

Anti-KAT1 / HAT1 antibody
See all KAT1 / HAT1 products (5) ...

Description

Rabbit polyclonal to KAT1 / HAT1

Specificity

ab12163 specifically recognizes human HAT1.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Chicken, Human

Immunogen

Synthetic peptide: EESFQELVEDYRRVIERLAQE with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1. The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).

EESFQELVED YRRVIERLAQ E

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Whole extracts of human 293 cells, mouse NIH-3T3 cells, or chicken fibroblasts.

General notes

Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

Applications

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Our Abpromise guarantee covers the use of ab12163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.

Sequence similarities

Belongs to the HAT1 family.

Developmental stage

Highly expressed in mitotic cells (at protein level).

Cellular localization

Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.

Target information above from: UniProt accessionO14929 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HAT 1 antibody
  • hat1 antibody
  • HAT1_HUMAN antibody
  • Histidine aminotransferase 1 antibody
  • Histone acetyltransferase 1 antibody
  • Histone acetyltransferase type B catalytic subunit antibody
  • KAT1 antibody
see all

Database links

References for Anti-KAT1 / HAT1 antibody (ab12163)

ab12163 has not yet been referenced specifically in any publications.

Publishing research using ab12163? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"