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Product Name 

KAT1 / HAT1 antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to KAT1 / HAT1

Immunogen 

Synthetic peptide: EESFQELVEDYRRVIERLAQE with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1.
The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).
BLAST 'EESFQELVEDYRRVIERLAQE' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Chk

Specificity 

ab12163 specifically recognizes human HAT1.

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: 1/2500. Detects a band of approximately 46 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole extracts of human 293 cells, mouse NIH-3T3 cells, or chicken fibroblasts.

Cellular localization 

Nuclear in S-phase cells and cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT

Relevance 

KAT1 / HAT1 is a histone acetyl transferase that is comprised of the 46kDa catalytic KAT1 and the accessory p46. KAT1 is known to acetylate histone H4 at K5 and K12.

 

Alternative names KAT1 / HAT1 antibody (ab12163)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

374037    (Chicken)
8520    (Human)
107435    (Mouse)

GeneCard

    

GC02P172742    (Human)

Omim

    

603053    (Human)

SwissProt

    

O14929    (Human)
Q8BY71    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Form 

Liquid

Concentration 

0.800 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAT1 / HAT1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to KAT1 / HAT1

 

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Loading controls

Lysates

 

Positive Controls for KAT1 / HAT1 antibody

ab7902

  

HEK293 Human embryonic kidney cell line Whole Cell Lysate

 

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