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Overview
Product name
Anti-KAT1 / HAT1 antibody
See all KAT1 / HAT1 products (5) ...
Description
Rabbit polyclonal to KAT1 / HAT1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: EKFLVEYKSAVEKK, with N-terminal added cysteine, conjugated to KLH, corresponding to amino acids 8-21 of Human HAT1.
EKFLVEYKSA VEKK
Positive control
Whole extracts of human 293 cells or mouse NIH-3T3 cells.
General notes
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/500, a chemiluminescent detection reagent is recommended. Predicted molecular weight: 46 kDa. In some preparations, an additional 1-2 bands (~90/30 kDa) may be detected.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Sequence similarities
Belongs to the HAT1 family.
Developmental stage
Highly expressed in mitotic cells (at protein level).
Cellular localization
Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
Target information above from: UniProt accessionO14929
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HAT 1 antibody
- hat1 antibody
- HAT1_HUMAN antibody
see all
Database links
- Entrez Gene: 8520 Human
- Entrez Gene: 107435 Mouse
- Entrez Gene: 296501 Rat
- Omim: 603053 Human
- SwissProt: O14929 Human
- SwissProt: Q8BY71 Mouse
- SwissProt: Q5M939 Rat
- Unigene: 632532 Human
see all
References for Anti-KAT1 / HAT1 antibody (ab12164)
This product has been referenced in:
- Alvarez Fet al. Sequential establishment of marks on soluble histones h3 and h4. J Biol Chem 286:17714-21 (2011). WB; Human.Read more (PubMed: 21454524) »
- Chekhun VFet al. Epigenetic profiling of multidrug-resistant human MCF-7 breast adenocarcinoma cells reveals novel hyper- and hypomethylated targets. Mol Cancer Ther 6:1089-98 (2007). WB; Human.Read more (PubMed: 17363502) »
See all 2 publications for this product
Publishing research using ab12164? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"