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Overview
Product name
Anti-KAT1 / HAT1 antibody
See all KAT1 / HAT1 products (5) ...
Description
Rabbit polyclonal to KAT1 / HAT1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal region of human HAT1.
Positive control
Extracts from LOVO cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - KAT1 / HAT1 antibody (ab61225)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Target
Function
Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Sequence similarities
Belongs to the HAT1 family.
Developmental stage
Highly expressed in mitotic cells (at protein level).
Cellular localization
Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
Target information above from: UniProt accessionO14929
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HAT 1 antibody
- hat1 antibody
- HAT1_HUMAN antibody
see all
Database links
- Entrez Gene: 8520 Human
- Entrez Gene: 107435 Mouse
- Entrez Gene: 296501 Rat
- Omim: 603053 Human
- SwissProt: O14929 Human
- SwissProt: Q8BY71 Mouse
- SwissProt: Q5M939 Rat
- Unigene: 632532 Human
see all
Anti-KAT1 / HAT1 antibody images:
Western blot - KAT1 / HAT1 antibody (ab61225)
All lanes : Anti-KAT1 / HAT1 antibody (ab61225) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide
Predicted band size : 49 kDa
Observed band size : 49 kDa
References for Anti-KAT1 / HAT1 antibody (ab61225)
This product has been referenced in:
- Ejlassi-Lassallette Aet al. H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo. Mol Biol Cell 22:245-55 (2011). WB; Other.Read more (PubMed: 21118997) »
See 1 publication for this product
Publishing research using ab61225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"