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Product name
Anti-KAT13A / SRC1 antibody - ChIP Grade
See all KAT13A / SRC1 products (6) ...
Description
Rabbit polyclonal to KAT13A / SRC1 - ChIP Grade
Specificity
Detects steroid receptor coactivator-1 (SRC-1).
Tested applications
IHC-Fr, WB, IHC-P, ChIP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: SSDPANPDSHKRKGSPC, corresponding to amino acids 8-24 of Human SRC1.
SSDPANPDSHKRKGSPC
Positive control
HeLa cell lysate
General notes
Locus Link: 8648
Alternative names: SRC1, NCoA-1, F-SRC-1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP
ICC/IF
Three stars (1 Abreview)
Application notes
ChIP: Use at an assay dependent dilution (PMID 19005469).
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Tissue specificity
Widely expressed.
Involvement in disease
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Domain
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
Post-translational
modifications
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15788
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NY REN 52 antigen antibody
- Protein Hin 2 antibody
- SRC 1 antibody
see all
Database links
- Entrez Gene: 8648 Human
- Entrez Gene: 17977 Mouse
- Omim: 602691 Human
- SwissProt: Q53SX3 Human
- SwissProt: Q15788 Human
- SwissProt: Q66JL7 Mouse
- SwissProt: P70365 Mouse
- Unigene: 412293 Human
see all
Anti-KAT13A / SRC1 antibody - ChIP Grade images:
Immunocytochemistry - KAT13A / SRC1 antibody (ab2859)
Immunolocalization of SRC-1 in HeLa cells using ab2859.
Immunohistochemistry (Frozen sections) - SRC1 antibody (ab2859)
ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
References for Anti-KAT13A / SRC1 antibody - ChIP Grade (ab2859)
This product has been referenced in:
- Hurtado A et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP; Human.Read more (PubMed: 19005469) »
- Koeberle PD & Bähr M The upregulation of GLAST-1 is an indirect antiapoptotic mechanism of GDNF and neurturin in the adult CNS. Cell Death Differ 15:471-83 (2008). WB.Read more (PubMed: 18064044) »
See all 3 publications for this product
Publishing research using ab2859? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"