Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-KAT13A / SRC1 antibody
See all KAT13A / SRC1 products (6) ...
Description
Rabbit polyclonal to KAT13A / SRC1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Immunogen was a 37 amino acid synthetic peptide, which represented a portion of human nuclear receptor coactivator 1 encoded within exon 23 (LocusLink ID 8648).
Positive control
Nuclear extract from HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance of 1.4 at 280 nm equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at a concentration of 1 - 4 µg/mg lysate.
Western blot: Not recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Tissue specificity
Widely expressed.
Involvement in disease
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Domain
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
Post-translational
modifications
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15788
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NY REN 52 antigen antibody
- Protein Hin 2 antibody
- SRC 1 antibody
see all
Database links
- Entrez Gene: 8648 Human
- Entrez Gene: 17977 Mouse
- Omim: 602691 Human
- SwissProt: Q53SX3 Human
- SwissProt: Q15788 Human
- SwissProt: Q66JL7 Mouse
- SwissProt: P70365 Mouse
- Unigene: 412293 Human
see all
Anti-KAT13A / SRC1 antibody images:
Immunoprecipitation - KAT13A / SRC1 antibody (ab10308)
ab10308 Immunoprecipitate KAT13A / SRC1 in human Huh-7 whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4°C. For western blotting an undiluted Abcam`s ab2859, Rabbit polyclonal to KAT13A / SRC1 was used.
This image is a courtesy of Anonymous Abreview
Immunoprecipitation - KAT13A / SRC1 antibody (ab10308)
ab10308 used in Immunoprecipitation.Total protein input is 1 x 106 cells.Whole cell lysate was prepared from VERO cells expressing recombinant SRC1 protein.Protein A matrix was used for the Immunoprecipitation step.ab10308 used a 4µg/mg lysate for 16 hours at 4°C.ab2859 was then used for Western Blot at 1µg/mg lysate.
Image courtesy of an anonymous Abreview.
References for Anti-KAT13A / SRC1 antibody (ab10308)
ab10308 has not yet been referenced specifically in any publications.
Publishing research using ab10308? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"