![Immunohistochemistry (Paraffin-embedded sections) - NCOA3 antibody [0.T.198] (ab14139) image](#ab14139_1.jpg)
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Overview
Product name
Anti-KAT13B / AIB1 antibody [0.T.198]
See all KAT13B / AIB1 products (10) ...
Description
Mouse monoclonal [0.T.198] to KAT13B / AIB1
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein, corresponding to amino acids 605-1294 of Human NCOA3.
Positive control
RIPA lysates from BG-1 ovarian cancer cells.
General notes
Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
0.T.198
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Immunohistochemistry (Paraffin-embedded sections) - NCOA3 antibody [0.T.198] (ab14139)
![Immunohistochemistry (Paraffin-embedded sections) - NCOA3 antibody [0.T.198] (ab14139) image](/ps/datasheet/Images/14/ab14139/ab14139_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 150 kDa (predicted molecular weight: 170 kDa).
IP: Use 10µl for 10500 cells.
IHC-P: Use at an assay dependent dilution.
Target
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
Tissue specificity
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Domain
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
Post-translational
modifications
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function.
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Target information above from: UniProt accessionQ9Y6Q9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTR antibody
- AIB 1 antibody
- AIB-1 antibody
see all
Database links
- Entrez Gene: 8202 Human
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- Unigene: 592142 Human
Anti-KAT13B / AIB1 antibody [0.T.198] images:
Immunohistochemistry (Paraffin-embedded sections) - NCOA3 antibody [0.T.198] (ab14139)
Superficial spreading type level IV malignant melanoma from the skin of the back, with microsatellite formation, possible lymphatic vascular invasion, low mitotic rate and moderate tumor infiltrating lymphocytes. Ab14139 used at a dilution of 1:25.
References for Anti-KAT13B / AIB1 antibody [0.T.198] (ab14139)
This product has been referenced in:
- Gould Rothberg BEet al. Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis. J Natl Cancer Inst 101:452-74 (2009).Read more (PubMed: 19318635) »
- Rangel Jet al. Prognostic significance of nuclear receptor coactivator-3 overexpression in primary cutaneous melanoma. J Clin Oncol 24:4565-9 (2006). IHC-P; Human.Read more (PubMed: 17008696) »
See all 2 publications for this product
Publishing research using ab14139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"