KAT13B / AIB1 antibody [0.T.198] (ab14139)

Product Name 

KAT13B / AIB1 antibody [0.T.198]

Product type 

Primary antibodies

Description 

Mouse monoclonal [0.T.198] to KAT13B / AIB1

Immunogen 

Fusion protein, corresponding to amino acids 605-1294 of Human NCOA3.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IP: Use 10µl for 10⁵⁰⁰ cells.
WB: 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 170 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

RIPA lysates from BG-1 ovarian cancer cells.

Cellular localization 

Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

KAT13B / AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits KAT3A / KAT3B as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

0.T.198

Isotype 

IgG

Purity 

Ascites

Storage buffer 

Preservative: 0.05% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

Notes 

Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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KAT13B / AIB1 antibody [0.T.198] images:

References for KAT13B / AIB1 antibody [0.T.198] (ab14139)

  This product has been used in: (two most recent references)

Gould Rothberg BE et al. Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis. J Natl Cancer Inst 101:452-74 (2009). PubMed: 19318635

Rangel J et al. Prognostic significance of nuclear receptor coactivator-3 overexpression in primary cutaneous melanoma. J Clin Oncol 24:4565-9 (2006). IHC-P; Human. PubMed: 17008696
If you publish research using ab14139 please let us know so that we can cite the reference on this datasheet.

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