Anti-SRC3 antibody [0.T.198] (ab14139)
- Product nameAnti-SRC3 antibody [0.T.198]See all SRC3 primary antibodies ...
- DescriptionMouse monoclonal [0.T.198] to SRC3
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Human
Fusion protein, corresponding to amino acids 605-1294 of Human NCOA3.
- Positive controlRIPA lysates from BG-1 ovarian cancer cells.
- General notesAliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
- Concentration information loading...
- Clonality Monoclonal
- Clone number0.T.198
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
Our Abpromise guarantee covers the use of ab14139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 170 kDa).|
|IP||IP: Use 10µl for 10500 cells.|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
- FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
- Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
- Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
- DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
modificationsAcetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function.
- Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
- ACTR antibodyAIB 1 antibodyAIB-1 antibody
- AIB1 antibodyAmplified in breast cancer 1 antibodyAmplified in breast cancer 1 protein antibodybHLHe42 antibodyCAGH 16 antibodyCAGH16 antibodyCBP interacting protein antibodyCBP-interacting protein antibodyClass E basic helix-loop-helix protein 42 antibodyCTG 26 antibodyCTG26 antibodyKAT13B / AIB1 antibodyMGC141848 antibodyNCOA 3 antibodyNCOA 3 antibodyNCoA-3 antibodyNCOA3 antibodyNCOA3_HUMAN antibodyNuclear receptor coactivator 3 antibodyNuclear receptor coactivator 3 antibodypCIP antibodypCIP antibodyRAC 3 antibodyRAC-3 antibodyRAC3 antibodyReceptor associated coactivator 3 antibodyReceptor-associated coactivator 3 antibodySRC 3 antibodySRC-3 antibodySRC3 antibodySteroid receptor coactivator protein 3 antibodySteroid receptor coactivator protein 3 antibodyThyroid hormone receptor activator molecule 1 antibodyThyroid hormone receptor activator molecule 1 antibodyTNRC14 antibodyTNRC16 antibodyTRAM 1 antibodyTRAM-1 antibodyTRAM1 antibody
Anti-SRC3 antibody [0.T.198] images
Superficial spreading type level IV malignant melanoma from the skin of the back, with microsatellite formation, possible lymphatic vascular invasion, low mitotic rate and moderate tumor infiltrating lymphocytes. Ab14139 used at a dilution of 1:25.
References for Anti-SRC3 antibody [0.T.198] (ab14139)
This product has been referenced in:
- Gould Rothberg BE et al. Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis. J Natl Cancer Inst 101:452-74 (2009). Read more (PubMed: 19318635) »
- Rangel J et al. Prognostic significance of nuclear receptor coactivator-3 overexpression in primary cutaneous melanoma. J Clin Oncol 24:4565-9 (2006). IHC-P ; Human . Read more (PubMed: 17008696) »