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Anti-SRC3 antibody [AX 15] (ab12074)

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Overview

  • Product nameAnti-SRC3 antibody [AX 15]See all SRC3 primary antibodies ...
  • Description
    Mouse monoclonal [AX 15] to SRC3
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein containing amino acids 605-1294 of human NCOA3.

  • Positive controlBG-1 ovarian cell RIPA lysate.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Applications

Our Abpromise guarantee covers the use of ab12074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP
WB
  • Application notesIP: 15µl of ab12074 will immunoprecipitate NCOA3 from 0.5-1 mg of a BG-1 ovarian cell RIPA lysate.
    WB: 1/500. Ab12074 recognizes NCOA3 (150 kD) and another doublet (110 kD) by Western blotting a BG-1 ovarian cell RIPA lysate.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
    • Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
    • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
    • Post-translational
      modifications      Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
      Methylated by CARM1.
      Phosphorylated by IKK complex. Regulated its function.
    • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

      • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          ACTR antibodyAIB 1 antibodyAIB-1 antibody
          AIB1 antibodyAmplified in breast cancer 1 antibodyAmplified in breast cancer 1 protein antibodybHLHe42 antibodyCAGH 16 antibodyCAGH16 antibodyCBP interacting protein antibodyCBP-interacting protein antibodyClass E basic helix-loop-helix protein 42 antibodyCTG 26 antibodyCTG26 antibodyKAT13B / AIB1 antibodyMGC141848 antibodyNCOA 3 antibodyNCOA 3 antibodyNCoA-3 antibodyNCOA3 antibodyNCOA3_HUMAN antibodyNuclear receptor coactivator 3 antibodyNuclear receptor coactivator 3 antibodypCIP antibodypCIP antibodyRAC 3 antibodyRAC-3 antibodyRAC3 antibodyReceptor associated coactivator 3 antibodyReceptor-associated coactivator 3 antibodySRC 3 antibodySRC-3 antibodySRC3 antibodySteroid receptor coactivator protein 3 antibodySteroid receptor coactivator protein 3 antibodyThyroid hormone receptor activator molecule 1 antibodyThyroid hormone receptor activator molecule 1 antibodyTNRC14 antibodyTNRC16 antibodyTRAM 1 antibodyTRAM-1 antibodyTRAM1 antibody
        see all

      References for Anti-SRC3 antibody [AX 15] (ab12074)

      ab12074 has not yet been referenced specifically in any publications.

      Publishing research using ab12074 ? Please let us know so that we can cite the reference in this datasheet

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      I am interested in purchasing the BG-1 cell lysate that was used as the positive control for this Ab, but I do not see it listed on your website. Do you offer it? If not, do you have any idea who might?

      No we do not sell this lysate. You could try looking in Sigma's catalogue or perhaps searching for other providers on Biocompare.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"