![Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782) image](#ab2782_1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-KAT13B / AIB1 antibody [AX15.3] - ChIP Grade
See all KAT13B / AIB1 products (10) ...
Description
Mouse monoclonal [AX15.3] to KAT13B / AIB1 - ChIP Grade
Tested applications
ChIP, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein encoding amino acid residues 605-1294 of human AIB 1.
General notes
Locus Link: 8202.
Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1.
Alias: Amplified in breast cancer-1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
AX15.3
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
-
Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782)
![Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782) image](/ps/datasheet/Images/2/ab2782/ab2782_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IP: Use at an assay dependent dilution.
Target
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
Tissue specificity
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Domain
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
Post-translational
modifications
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function.
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Target information above from: UniProt accessionQ9Y6Q9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTR antibody
- AIB 1 antibody
- AIB-1 antibody
see all
Database links
- Entrez Gene: 8202 Human
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- Unigene: 592142 Human
Anti-KAT13B / AIB1 antibody [AX15.3] - ChIP Grade images:
Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782)
Predicted band size : 155 kDa
Western blot of AIB1 on MCF-7 cell extract using ab2782.
References for Anti-KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782)
This product has been referenced in:
- Levy Net al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP; Human.Read more (PubMed: 17962382) »
- Levy Net al. Multiple transcription factor elements collaborate with estrogen receptor alpha to activate an inducible estrogen response element in the NKG2E gene. Endocrinology 148:3449-58 (2007).Read more (PubMed: 17395694) »
See all 3 publications for this product
Publishing research using ab2782? Please let us know so that we can cite the reference in this datasheet
![KAT13B / AIB1 antibody [AX15.3] - ChIP Grade for WB in Human (2782)](/ps/datasheet/images/2/ab2782/KAT13B-AIB1-Primary-antibodies-ab2782-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"