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Overview

  • Product nameAnti-SRC3 antibody [AX15.3] - ChIP GradeSee all SRC3 primary antibodies ...
  • Description
    Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
  • Tested applicationsChIP, WB, IP more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein encoding amino acid residues 605-1294 of human AIB 1.

  • General notesLocus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.

Applications

Our Abpromise guarantee covers the use of ab2782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ChIP ChIP: Use at an assay dependent dilution.
WB WB: Use at an assay dependent dilution. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IP IP: Use at an assay dependent dilution.

Target

  • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • Post-translational
    modifications    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

    • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ACTR antibodyAIB 1 antibodyAIB-1 antibody
        AIB1 antibodyAmplified in breast cancer 1 antibodyAmplified in breast cancer 1 protein antibodybHLHe42 antibodyCAGH 16 antibodyCAGH16 antibodyCBP interacting protein antibodyCBP-interacting protein antibodyClass E basic helix-loop-helix protein 42 antibodyCTG 26 antibodyCTG26 antibodyKAT13B / AIB1 antibodyMGC141848 antibodyNCOA 3 antibodyNCoA-3 antibodyNCOA3 antibodyNCOA3_HUMAN antibodyNuclear receptor coactivator 3 antibodyNuclear receptor coactivator 3 antibodypCIP antibodyRAC 3 antibodyRAC-3 antibodyRAC3 antibodyReceptor associated coactivator 3 antibodyReceptor-associated coactivator 3 antibodySRC 3 antibodySRC-3 antibodySRC3 antibodySteroid receptor coactivator protein 3 antibodySteroid receptor coactivator protein 3 antibodyThyroid hormone receptor activator molecule 1 antibodyThyroid hormone receptor activator molecule 1 antibodyTNRC14 antibodyTNRC16 antibodyTRAM 1 antibodyTRAM-1 antibodyTRAM1 antibody
      see all

    Anti-SRC3 antibody [AX15.3] - ChIP Grade images

    • Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782)
      Western blot - KAT13B / AIB1 antibody [AX15.3] - ChIP Grade (ab2782)


      Predicted band size : 155 kDa


      Western blot of AIB1 on MCF-7 cell extract using ab2782.

    References for Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

    This product has been referenced in:
    • Levy N  et al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP ; Human . Read more (PubMed: 17962382) »
    • Levy N  et al. Multiple transcription factor elements collaborate with estrogen receptor alpha to activate an inducible estrogen response element in the NKG2E gene. Endocrinology 148:3449-58 (2007). Read more (PubMed: 17395694) »

    See all 3 Publications for this product

    Publishing research using ab2782 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-SRC3 antibody [AX15.3] - ChIP Grade

     Good
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 20000 cells
    Specification huh-7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Submitted Jun 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"