Anti-SRC3 antibody - ChIP Grade (ab2831)

Overview

• Product nameAnti-SRC3 antibody - ChIP GradeSee all SRC3 primary antibodies ...
• Description
  Rabbit polyclonal to SRC3 - ChIP Grade
• SpecificityDetects amplified in breast cancer 1 (AIB1).
• Tested applicationsWB, IHC-P, ChIP more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Xenopus laevis
• Immunogen

  Synthetic peptide: GLGENLDPLASDSC, corresponding to amino acids 3-15 of Human AIB 1.

  Run BLAST with Run BLAST with
• Positive controlT47D cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS, 1 mg/ml BSA, 0.05% sodium azide
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Coactivators and repressors

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

Applications

Target

• FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
• Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
• Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
  Contains 1 basic helix-loop-helix (bHLH) domain.
  Contains 1 PAS (PER-ARNT-SIM) domain.
• DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
• Post-translational
  modificationsAcetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
  Methylated by CARM1.
  Phosphorylated by IKK complex. Regulated its function.
• Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8202 Human
  • Entrez Gene: 8202 Human
  • Entrez Gene: 398021 Xenopus laevis
  • Omim: 601937 Human
  • SwissProt: Q9Y6Q9 Human
  • SwissProt: Q9Y6Q9 Human
  • SwissProt: O57539 Xenopus laevis
  • Unigene: 592142 Human

  • Unigene: 268 Xenopus laevis

  see all

• Alternative names
  ACTR antibodyAIB 1 antibodyAIB-1 antibody

  AIB1 antibodyAmplified in breast cancer 1 antibodyAmplified in breast cancer 1 protein antibodybHLHe42 antibodyCAGH 16 antibodyCAGH16 antibodyCBP interacting protein antibodyCBP-interacting protein antibodyClass E basic helix-loop-helix protein 42 antibodyCTG 26 antibodyCTG26 antibodyKAT13B / AIB1 antibodyMGC141848 antibodyNCOA 3 antibodyNCoA-3 antibodyNCOA3 antibodyNCOA3_HUMAN antibodyNuclear receptor coactivator 3 antibodyNuclear receptor coactivator 3 antibodypCIP antibodyRAC 3 antibodyRAC-3 antibodyRAC3 antibodyReceptor associated coactivator 3 antibodyReceptor-associated coactivator 3 antibodySRC 3 antibodySRC-3 antibodySRC3 antibodySteroid receptor coactivator protein 3 antibodySteroid receptor coactivator protein 3 antibodyThyroid hormone receptor activator molecule 1 antibodyThyroid hormone receptor activator molecule 1 antibodyTNRC14 antibodyTNRC16 antibodyTRAM 1 antibodyTRAM-1 antibodyTRAM1 antibody

  see all

Anti-SRC3 antibody - ChIP Grade images

• 

  ChIP - KAT13B / AIB1 antibody - ChIP Grade (ab2831)Sylvain Daujat

  ab2831 can be used in ChIP. You can expect recruitment of AIB1on the pS2 promoter at some point during transcriptional activation. The amount of DNA precipiated is significant.

  Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab2831 to AIB1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab2831 and 2x106 cells were used in each ChIP experiment. 
• 

  Immunocytochemistry/ Immunofluorescence - KAT13B / AIB1 antibody - ChIP Grade (ab2831)

  ICC/IF image of ab2831 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2831, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-KAT13B / AIB1 antibody - ChIP Grade(ab2831)
  IHC image of ab2831 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2831, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.