|
|
|
|
ChIP - KAT13B / AIB1 antibody - ChIP Grade (ab2831)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - KAT13B / AIB1 antibody - ChIP Grade (ab2831)
(enlarge)
|
|
|
|
|
Product Name
|
KAT13B / AIB1 antibody - ChIP Grade
|
| |
See all KAT13B / AIB1 antibodies (10)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to KAT13B / AIB1 - ChIP Grade
|
|
Immunogen
|
Synthetic peptide: GLGENLDPLASDSC, corresponding to amino acids 3-15 of Human AIB 1.
BLAST 'GLGENLDPLASDSC' with  or 
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Xl
(Due to sequence homology)
|
|
Specificity
|
Detects amplified in breast cancer 1 (AIB1).
|
|
Tested applications
(see key)
|
ChIP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use 3µg for 106 cells.
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 155 kDa. Can be blocked with AIB 1 peptide or re-use.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
T47D cell lysate
|
|
Cellular localization
|
Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
|
|
Relevance
|
KAT13B / AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits KAT3A / KAT3B as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAT13B / AIB1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for KAT13B / AIB1 antibody - ChIP Grade images, references, products related to ab2831 and other tools.
|
| |
KAT13B / AIB1 antibody - ChIP Grade images:
|
 ChIP - KAT13B / AIB1 antibody - ChIP Grade (ab2831)
ab2831 can be used in ChIP. You can expect recruitment of AIB1on the pS2 promoter at some point during transcriptional activation. The amount of DNA precipiated is significant.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab2831 to AIB1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab2831 and 2x106 cells were used in each ChIP experiment.
Sylvain Daujat
|
| |
Immunocytochemistry/ Immunofluorescence - KAT13B / AIB1 antibody - ChIP Grade (ab2831)
ICC/IF image of ab2831 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2831, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
|
| |
References for KAT13B / AIB1 antibody - ChIP Grade (ab2831)
|
|
This product has been used in: (two most recent references)
Mason CE et al. Location analysis for the estrogen receptor-{alpha} reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res : (2010). ChIP; Human. PubMed: 20047966
Fenne IS et al. Recruitment of Coactivator GRIP1 to an Estrogen Receptor Transcription Complex is Regulated by the cAMP-dependent Protein Kinase. Endocrinology : (2008). ChIP; Human. PubMed: 18499756
See all 3 publication references for this product.
If you publish research using ab2831 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to KAT13B / AIB1
|
| |
KAT13B / AIB1 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding KAT13B / AIB1 antibody - ChIP Grade |
Customer FAQs regarding KAT13B / AIB1 antibody - ChIP Grade |
Protocols for KAT13B / AIB1 antibody - ChIP Grade |
Price and availability of products related to KAT13B / AIB1 antibody - ChIP Grade |
| |
KAT13B / AIB1 antibody - ChIP Grade - related products:
|
|
|
| |
KAT13B / AIB1 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about KAT13B / AIB1 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
