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Overview
Product name
Anti-KAT13B / AIB1 antibody
See all KAT13B / AIB1 products (10) ...
Description
Rabbit polyclonal to KAT13B / AIB1
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at a concentration of 20 µg/10mg extract.
WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
Tissue specificity
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
Domain
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
Post-translational
modifications
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function.
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Target information above from: UniProt accessionQ9Y6Q9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTR antibody
- ACTR antibody
- AIB 1 antibody
see all
Database links
- Entrez Gene: 8202 Human
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- Unigene: 592142 Human
Anti-KAT13B / AIB1 antibody images:
Immunoprecipitation - KAT13B / AIB1 antibody (ab10310)
Detection of SCR3 by Immunoprecipitation.
Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20
Western blot - KAT13B / AIB1 antibody (ab10310)
Predicted band size : 170 kDa
Detection of SCR3 by Immunoprecipitation.
Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.
References for Anti-KAT13B / AIB1 antibody (ab10310)
This product has been referenced in:
- Tilli MTet al. Overexpression of an N-terminally truncated isoform of the nuclear receptor coactivator amplified in breast cancer 1 leads to altered proliferation of mammary epithelial cells in transgenic mice. Mol Endocrinol 19:644-56 (2005). WB; Mouse.Read more (PubMed: 15550471) »
See 1 publication for this product
Publishing research using ab10310? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"