Anti-SRC3 antibody (ab85452)

Overview

• Product nameAnti-SRC3 antibodySee all SRC3 primary antibodies ...
• Description
  Rabbit polyclonal to SRC3
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Pig
• Immunogen

  Synthetic peptide corresponding to a region within the internal sequence amino acids 576-625 (DQNPVESSMC QSNSRDHLSD KESKESSVEG AENQRGPLES KGHKKLLQLL) of human KAT13B / AIB1 (NP_006525).

  Run BLAST with Run BLAST with
• Positive control293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab85452 is purified by peptide affinity chromatography method.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Coactivators and repressors

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

Applications

Target

• FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
• Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
• Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
  Contains 1 basic helix-loop-helix (bHLH) domain.
  Contains 1 PAS (PER-ARNT-SIM) domain.
• DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
• Post-translational
  modificationsAcetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
  Methylated by CARM1.
  Phosphorylated by IKK complex. Regulated its function.
• Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8202 Human
  • Entrez Gene: 8202 Human
  • Entrez Gene: 100135659 Pig
  • Omim: 601937 Human
  • SwissProt: Q9Y6Q9 Human
  • SwissProt: Q9Y6Q9 Human
  • Unigene: 592142 Human

• Alternative names
  ACTR antibodyAIB 1 antibodyAIB-1 antibody

  AIB1 antibodyAmplified in breast cancer 1 antibodyAmplified in breast cancer 1 protein antibodybHLHe42 antibodyCAGH 16 antibodyCAGH16 antibodyCBP interacting protein antibodyCBP-interacting protein antibodyClass E basic helix-loop-helix protein 42 antibodyCTG 26 antibodyCTG26 antibodyKAT13B / AIB1 antibodyMGC141848 antibodyNCOA 3 antibodyNCoA-3 antibodyNCOA3 antibodyNCOA3_HUMAN antibodyNuclear receptor coactivator 3 antibodypCIP antibodyRAC 3 antibodyRAC-3 antibodyRAC3 antibodyReceptor associated coactivator 3 antibodyReceptor-associated coactivator 3 antibodySRC 3 antibodySRC-3 antibodySRC3 antibodySteroid receptor coactivator protein 3 antibodyThyroid hormone receptor activator molecule 1 antibodyTNRC14 antibodyTNRC16 antibodyTRAM 1 antibodyTRAM-1 antibodyTRAM1 antibody

  see all

Anti-SRC3 antibody images

• 

  Western blot - KAT13B / AIB1 antibody (ab85452)
  Anti-SRC3 antibody (ab85452) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 155 kDa
  Observed band size : 155 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SRC3 antibody(ab85452)
  Immunohistochemistry of Human Tonsil lysate tissue at an antibody concentration of 5.0µg/ml using anti-NCOA3 antibody (ab85452)