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Overview
Product name
Anti-KAT13C / NCOA2 antibody - ChIP Grade
See all KAT13C / NCOA2 products (11) ...
Description
Rabbit polyclonal to KAT13C / NCOA2 - ChIP Grade
Tested applications
IHC-P, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represents aportion between residues 600 and 700 of the human Nuclear Receptor Coactivator 2 (GenBank PID 1877215).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were affinity purified usingthe peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT13C / NCOA2 antibody - ChIP Grade (ab9261)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IP: Use a concentration of 1 - 4 µg/ml.
WB: 1/500 - 1/5000.Predicted molecular weight: 159 kDa.
ChIP: Use at an assay dependent dilution.
Target
Relevance
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is KAT13C / nuclear receptor coactivator 2 (NCOA2), also termed Glucocorticoid receptor-interacting protein 1 (GRIP1).
Cellular localization
Nuclear
Alternative names
- glucocorticoid receptor interacting protein 1 antibody
- GRIP 1 antibody
- GRIP1 antibody
see all
Database links
- Entrez Gene: 10499 Human
- Omim: 601993 Human
- SwissProt: Q15596 Human
- Unigene: 446678 Human
Anti-KAT13C / NCOA2 antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT13C / NCOA2 antibody - ChIP Grade (ab9261)
ab9261 (2µg/ml) staining KAT13C in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-KAT13C / NCOA2 antibody - ChIP Grade (ab9261)
This product has been referenced in:
- Cvoro Aet al. Cross Talk between Glucocorticoid and Estrogen Receptors Occurs at a Subset of Proinflammatory Genes. J Immunol 186:4354-60 (2011). ChIP; Human.Read more (PubMed: 21357268) »
- Levy Net al. Unliganded estrogen receptor-beta regulation of genes is inhibited by tamoxifen. Mol Cell Endocrinol 315:201-7 (2010).Read more (PubMed: 19744542) »
See all 6 publications for this product
Publishing research using ab9261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"