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10491 and/or 10508 (both are NCOA2 antibodies). As I understand from your product datasheets immunogen was (3 prime end of exon 13 (and exon 26, for second ab). Can you please tell me which amino acids do these region(s) correspond to. |
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ANSWER: |
The epitope recognized by ab10491 maps to a region between residues 750 and 800 of Swiss-Prot entry Q15596 (http://au.expasy.org/cgi-bin/niceprot.pl?Q15596). The epitope recognized by ab10508 maps to a region within the C-terminal 50 residues of Swiss-Prot entry Q15596 (http://au.expasy.org/cgi-bin/niceprot.pl?Q15596). |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 175 kDa
ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) for WB and 20 µg/10 mg lysate (B) for IP. Detection: Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min. (A) or Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2) (B).
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