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Overview
Product name
Anti-KAT13C / NCOA2 antibody
See all KAT13C / NCOA2 products (11) ...
Description
Rabbit polyclonal to KAT13C / NCOA2
Specificity
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human nuclear receptor coactivator 2 encoded near the 3 prime end of exon 13 (LocusLink ID 10499).
Epitope
Maps to a region between residues 750 and 800 of Swiss Prot entry Q15596.
Positive control
Nuclear extract from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Western blot - KAT13C / NCOA2 antibody (ab10491)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at a concentration of 1 - 4 µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is KAT13C / nuclear receptor coactivator 2 (NCOA2), also termed Glucocorticoid receptor-interacting protein 1 (GRIP1).
Cellular localization
Nuclear
Alternative names
- glucocorticoid receptor interacting protein 1 antibody
- GRIP 1 antibody
- GRIP1 antibody
see all
Database links
- Entrez Gene: 10499 Human
- Entrez Gene: 17978 Mouse
- Omim: 601993 Human
- SwissProt: Q15596 Human
- SwissProt: Q61026 Mouse
- Unigene: 446678 Human
Anti-KAT13C / NCOA2 antibody images:
Western blot - KAT13C / NCOA2 antibody (ab10491)
Predicted band size : 175 kDa
ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) for WB and 20 µg/10 mg lysate (B) for IP. Detection: Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min. (A) or Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2) (B).
References for Anti-KAT13C / NCOA2 antibody (ab10491)
This product has been referenced in:
- Amazit Let al. Ligand-Dependent Degradation of SRC-1 Is Pivotal for Progesterone Receptor Transcriptional Activity. Mol Endocrinol 25:394-408 (2011). WB; Human.Read more (PubMed: 21273440) »
- Jelcick ASet al. Genetic variations strongly influence phenotypic outcome in the mouse retina. PLoS One 6:e21858 (2011). WB; Mouse.Read more (PubMed: 21779340) »
See all 3 publications for this product
Publishing research using ab10491? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"