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Overview

Applications

Our Abpromise guarantee covers the use of ab5625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 101.5 kDa).Can be blocked with CLOCK peptide (ab5855).

Target

  • FunctionARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
  • Tissue specificityExpressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications    Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
  • Cellular localizationCytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.

    • Target information above from: UniProt accession O15516 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      bHLHe8 antibodyCircadian locomoter output cycles kaput protein antibodyCircadian locomoter output cycles protein kaput antibody
      Circadian Locomotor Output Cycles Kaput antibodyCircadian Locomotor Output Cycles Kaput antibodyCircadium Locomotor Output Cycles Kaput antibodyClass E basic helix-loop-helix protein 8 antibodyCLOCK antibodyClock homolog antibodyClock protein antibodyCLOCK_HUMAN antibodyhCLOCK antibodyKIAA0334 antibody
    see all

References for Anti-KAT13D / CLOCK antibody (ab5625)

ab5625 has not yet been referenced specifically in any publications.

Publishing research using ab5625 ? Please let us know so that we can cite the reference in this datasheet

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